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Literature summary extracted from

  • Lee, P.; Colman, R.F.
    Thr373, Asp375, and Lys260 are in the coenzyme site of porcine NADP-dependent isocitrate dehydrogenase (2006), Arch. Biochem. Biophys., 450, 183-190.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 D375N 15fold increase in KM-value for NADP+, marked decrease of Vmax-value Sus scrofa
1.1.1.42 K260Q 28fold increase in KM-value for NADP+, marked decrease of Vmax-value Sus scrofa
1.1.1.42 K374Q little change in kinetic parameters Sus scrofa
1.1.1.42 T373A reduction of Vmax-value to 1% of wild-type Sus scrofa
1.1.1.42 T373S little change in kinetic parameters Sus scrofa
1.1.1.42 T373V reduction of Vmax-value to 20% of wild-type Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0055
-
 NADP+ mutant T373S, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0062
-
 NADP+ mutant T373A, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0088
-
 NADP+ wild-type, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.012
-
 NADP+ mutant T373V, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0154
-
 NADP+ mutant K374Q 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0179
-
 NAD+ wild-type, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0207
-
 NAD+ mutant K260R, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0232
-
 NAD+ mutant D375N, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0312
-
 NAD+ mutant K260Q, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.0378
-
 NADP+ mutant K260R, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.133
-
 NADP+ mutant D375N, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.25
-
 NADP+ mutant K260Q, 25°C, pH 7.4 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Sus scrofa P33198
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 DL-isocitrate + NAD+
-
 Sus scrofa 2-oxoglutarate + CO2 + NADH
-
 ?
1.1.1.42 DL-isocitrate + NADP+
-
 Sus scrofa 2-oxoglutarate + CO2 + NADPH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 0.03
-
 NAD+ mutant D375N, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.47
-
 NAD+ mutant K260Q, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.5
-
 NAD+ mutant K260R, 25°C, pH 7.4 Sus scrofa
1.1.1.42 0.76
-
 NAD+ wild-type, 25°C, pH 7.4 Sus scrofa
1.1.1.42 1.7
-
 NADP+ mutant K260Q, 25°C, pH 7.4 Sus scrofa
1.1.1.42 5.7
-
 NADP+ mutant K260R, 25°C, pH 7.4 Sus scrofa
1.1.1.42 8.4
-
 NADP+ mutant D375N, 25°C, pH 7.4 Sus scrofa
1.1.1.42 32.2
-
 NADP+ wild-type, 25°C, pH 7.4 Sus scrofa