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Literature summary extracted from

  • Elisakova, V.; Patek, M.; Holatko, J.; Nesvera, J.; Leyval, D.; Goergen, J.L.; Delaunay, S.
    Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum (2005), Appl. Environ. Microbiol., 71, 207-213.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.86 gene ilvC expression in Escherichia coli using an Escherichia coli-Corynebacterium glutamicum shuttle vector pECKA Corynebacterium glutamicum
2.2.1.6 expression of wild-type and mutant enzymes via shuttle vector pECKA in Escherichia coli and in Corynebacterium glutamicum Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 additional information mutations of the residues M8 and M13 of the small, regulatory subunit encoded by gene ilvN result in reduced sensitivity of the mutant enzymes to feedback inhibition which leads to increased production of valine as well as of isoleucine and leucine Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 isoleucine feedback inhibition of wild-type enzyme about 50% at 10 mM, M8 and M13 mutants are resistant Corynebacterium glutamicum
2.2.1.6 leucine feedback inhibition of wild-type enzyme about 50% at 10 mM, M8 and M13 mutants are resistant Corynebacterium glutamicum
2.2.1.6 valine feedback inhibition of wild-type enzyme about 50% at 10 mM, M8 and M13 mutants are resistant Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+
-
 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH Corynebacterium glutamicum
-
 2,3-dihydroxy-3-methylvalerate + NADP+
-
 ?
1.1.1.86 2-acetolactate + NADPH Corynebacterium glutamicum
-
 2,3-dihydroxyisovalerate + NADP+
-
 ?
1.1.1.86 additional information Corynebacterium glutamicum the enzyme is involved in biosynthesis of branched amino acids valine, isoleucine, and leucine, pathway overview ?
-
 ?
2.2.1.6 pyruvate Corynebacterium glutamicum the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine, overview 2-acetolactate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Corynebacterium glutamicum
-
 strain ATCC 13032, gene ilvC
-
2.2.1.6 Corynebacterium glutamicum
-
 genes ilvBN of the ilvBNC operon, genes ilvB and ilvN encode the catalytic and the small, regulatory subunits, respectively
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH
-
 Corynebacterium glutamicum 2,3-dihydroxy-3-methylvalerate + NADP+
-
 ?
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH the enzyme is involved in biosynthesis of the branched chain amino acids valine and leucine, pathway overview Corynebacterium glutamicum 2,3-dihydroxy-3-methylvalerate + NADP+
-
 ?
1.1.1.86 2-acetolactate + NADPH
-
 Corynebacterium glutamicum 2,3-dihydroxyisovalerate + NADP+
-
 ?
1.1.1.86 2-acetolactate + NADPH the enzyme is involved in biosynthesis of the branched chain amino acid isoleucine, pathway overview Corynebacterium glutamicum 2,3-dihydroxyisovalerate + NADP+
-
 ?
1.1.1.86 additional information the enzyme is involved in biosynthesis of branched amino acids valine, isoleucine, and leucine, pathway overview Corynebacterium glutamicum ?
-
 ?
2.2.1.6 pyruvate
-
 Corynebacterium glutamicum 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine, overview Corynebacterium glutamicum 2-acetolactate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.86 acetohydroxy acid isomeroreductase
-
 Corynebacterium glutamicum
1.1.1.86 AHAIR
-
 Corynebacterium glutamicum
2.2.1.6 acetohydroxyacid synthase
-
 Corynebacterium glutamicum
2.2.1.6 AHAS
-
 Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.3
-
 assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Corynebacterium glutamicum
2.2.1.6 FAD
-
 Corynebacterium glutamicum
2.2.1.6 thiamine diphosphate
-
 Corynebacterium glutamicum