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Literature summary extracted from

  • Böer, E.; Wartmann, T.; Schmidt, S.; Bode, R.; Gellissen, G.; Kunze, G.
    Characterization of the AXDH gene and the encoded xylitol dehydrogenase from the dimorphic yeast Arxula adeninivorans (2005), Antonie van Leeuwenhoek, 87, 233-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 expression in Escherichia coli Blastobotrys adeninivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.9 0.7
-
 D-ribulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 0.9
-
 L-sorbose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 2
-
 D-xylulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 4
-
 xylitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 4
-
 D-sorbitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 5.2
-
 xylitol 35°C, pH 7.5, recombinant enzyme Blastobotrys adeninivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.9 40300
-
 2 * 40300, calculated from sequence Blastobotrys adeninivorans
1.1.1.9 72000
-
 native enzyme, gel filtration, non-denaturing PAGE Blastobotrys adeninivorans
1.1.1.9 80000
-
 recombinant enzyme, gel filtration, non-denaturing PAGE Blastobotrys adeninivorans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Blastobotrys adeninivorans Q6KAV2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9
-
 Blastobotrys adeninivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-ribulose + NADH + H+
-
 Blastobotrys adeninivorans ?
-
 ?
1.1.1.9 D-sorbitol + NAD+
-
 Blastobotrys adeninivorans D-fructose + NADH + H+
-
 ir
1.1.1.9 L-sorbose + NADH + H+
-
 Blastobotrys adeninivorans ?
-
 ?
1.1.1.9 ribitol + NAD+
-
 Blastobotrys adeninivorans ?
-
 ?
1.1.1.9 xylitol + NAD+
-
 Blastobotrys adeninivorans D-xylulose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.9 dimer 2 * 40300, calculated from sequence Blastobotrys adeninivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 35
-
 native and recombinant enzyme Blastobotrys adeninivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.9 74
-
 D-ribulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 216
-
 L-sorbose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 302.9
-
 D-xylulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 528
-
 D-sorbitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
1.1.1.9 2115
-
 xylitol 35°C, pH 7.5, recombinant enzyme Blastobotrys adeninivorans
1.1.1.9 2644
-
 xylitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 7.5
-
 native and recombinant enzyme Blastobotrys adeninivorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.9 7.2 7.9 stable Blastobotrys adeninivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+ no activity with NADP+ Blastobotrys adeninivorans
1.1.1.9 NADH no activity with NADPH Blastobotrys adeninivorans