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Literature summary extracted from

  • Aoki, K.; Tanaka, N.; Ishikura, S.; Araki, N.; Imamura, Y.; Hara, A.; Nakamura, K.T.
    Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase (2006), Acta Crystallogr. Sect. F, 62, 1037-1040.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Sus scrofa
1.1.1.184 expression in Escherichia coli strain BL21(DE3) Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 crystallization to crystal forms I and II in the presence of NADPH. Form I crystals belong to the tetragonal space group P42, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P41212, and diffract to 2.2 A resolution Sus scrofa
1.1.1.184 purified and dialyzed recombinant enzyme complexed with cofactor, 3 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 1 mM NADPH, hanging drop vapour diffusion method, 0.002 ml of protein and crystallization solutions are mixed at 20°C, and equilibrated against 0.5 ml reservoir solution, which contains 2.2 M sodium citrate and 20%(v/v) glycerol in 100 mM HEPES buffer, pH 7.5 or 1.4 M ammonium sulfate and 20%(v/v) glycerol in 100 mM MES buffer pH 6.0, resulting in two different tetragonal crystal forms, 1 week, X-ray diffraction structure determination and analysis at 1.5-2.2 A resolution Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.184 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 27000
-
 x * 27000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, and affinity and hydroxylapatite chromatography, followed by ultrafiltration, the protein easily precipitates at concentraions of above 3.3 mg/ml Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 additional information the enzyme catalyses the NADPH-linked reduction of a variety of carbonyl compounds to their secondary alcohols Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 ? x * 27000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 CR
-
 Sus scrofa
1.1.1.184 More the enzyme belongs to the short-chain dehydrogenase/reductase family Sus scrofa
1.1.1.184 PHCR
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH specific for Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.184 Sus scrofa calculated
-
 9.3
1.1.1.184 Sus scrofa isoelectric focusing, recombinant enzyme
-
 9.3