Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yao, M.; Yasutake, Y.; Morita, H.; Tanaka, I.
    Structure of the type I L-asparaginase from the hyperthermophilic archaeon Pyrococcus horikoshii at 2.16 A resolution (2005), Acta Crystallogr. Sect. D, D61, 294-301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 gene PH0066, expression in Escherichia coli strain BL21 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 purified recombinant enzyme, hanging drop vapour diffusion method, 0.002 ml or protein solution is mixed with 0.001 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 12% v/v PEG 400, and 30 mM NaCl, soaking of crystals in 25% v/v PEG 400 and flash-cooling, X-ray difraction structure determination and analysis at 2.16 A resolution Pyrococcus horikoshii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 cytosol
-
Pyrococcus horikoshii 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Pyrococcus horikoshii
-
L-aspartate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant PhA from Escherichia coli strain BL21 by anion exchange chromatography and gel filtration, followed by hydrophobic interaction chromatography and ultrafiltration Pyrococcus horikoshii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.1 L-asparagine + H2O = L-aspartate + NH3 active site structure with a flexible loop that is stabilized by a beta-hairpin formation and intracellular interaction with the alpha-helical region, modelling Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
Pyrococcus horikoshii L-aspartate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer homodimer, crystal structure Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparagine aminohydrolase
-
Pyrococcus horikoshii
3.5.1.1 PhA
-
Pyrococcus horikoshii
3.5.1.1 type I L-asparaginase
-
Pyrococcus horikoshii