EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.1 | gene PH0066, expression in Escherichia coli strain BL21 | Pyrococcus horikoshii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.1 | purified recombinant enzyme, hanging drop vapour diffusion method, 0.002 ml or protein solution is mixed with 0.001 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 12% v/v PEG 400, and 30 mM NaCl, soaking of crystals in 25% v/v PEG 400 and flash-cooling, X-ray difraction structure determination and analysis at 2.16 A resolution | Pyrococcus horikoshii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.1 | cytosol | - |
Pyrococcus horikoshii | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | Pyrococcus horikoshii | - |
L-aspartate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Pyrococcus horikoshii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.1 | recombinant PhA from Escherichia coli strain BL21 by anion exchange chromatography and gel filtration, followed by hydrophobic interaction chromatography and ultrafiltration | Pyrococcus horikoshii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O = L-aspartate + NH3 | active site structure with a flexible loop that is stabilized by a beta-hairpin formation and intracellular interaction with the alpha-helical region, modelling | Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Pyrococcus horikoshii | L-aspartate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.1 | dimer | homodimer, crystal structure | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.1 | L-asparagine aminohydrolase | - |
Pyrococcus horikoshii |
3.5.1.1 | PhA | - |
Pyrococcus horikoshii |
3.5.1.1 | type I L-asparaginase | - |
Pyrococcus horikoshii |