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Literature summary extracted from
- Purification and properties of hyaluronidase from Palamneus gravimanus (Indian black scorpion) venom (2006), Toxicon, 47, 188-195.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.35 | NaCl | activates at 0.15 M | Palamneus gravimanus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.35 | NaCl stabilizes at 0.15 M | Palamneus gravimanus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.35 | 2-mercaptoethanol | - |
Palamneus gravimanus | |
| 3.2.1.35 | DTT | - |
Palamneus gravimanus | |
| 3.2.1.35 | EDTA | - |
Palamneus gravimanus | |
| 3.2.1.35 | glutathione | - |
Palamneus gravimanus | |
| 3.2.1.35 | heparin | - |
Palamneus gravimanus | |
| 3.2.1.35 | L-cysteine | - |
Palamneus gravimanus | |
| 3.2.1.35 | Urea | - |
Palamneus gravimanus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.35 | 0.048 | - |
hyaluronan | pH 5.0, 37°C | Palamneus gravimanus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.35 | extracellular | venom | Palamneus gravimanus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 52000 | - |
gel filtration | Palamneus gravimanus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | Palamneus gravimanus | - |
Indian black scorpion | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.35 | 25.6fold to homogeneity from venom by gel filtration and ion exchange chromatography | Palamneus gravimanus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | venom | - |
Palamneus gravimanus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 6412 | - |
turbidity reduction units/min/mg, purified enzyme | Palamneus gravimanus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.35 | -20°C, purified enzyme, 0.15 M NaCl, stable for 2 months | Palamneus gravimanus |
| 3.2.1.35 | 4°C, purified enzyme, 0.15 M NaCl, stable for 1 month | Palamneus gravimanus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.35 | hyaluronan + H2O | - |
Palamneus gravimanus | hyaluronan oligomers | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | monomer | 1 * 52000, SDS-PAGE | Palamneus gravimanus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | hyaluronidase | - |
Palamneus gravimanus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 37 | - |
- |
Palamneus gravimanus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 30 | 40 | stable, purified enzyme | Palamneus gravimanus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 4.5 | - |
- |
Palamneus gravimanus |
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