Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xue, S.; Calvin, K.; Li, H.
    RNA recognition and cleavage by a splicing endonuclease (2006), Science, 312, 906-910.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.16 Archaeoglobus fulgidus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.16 pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus substrate recognition and binding structure and mechanism, catalytic mechanism, the catalytic triad is formed by His257, Lys287, and Tyr246, overview Archaeoglobus fulgidus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.16 additional information the enzyme cleaves two phosphodiester bonds within folded precursor RNAs during intron removal producing functional RNAs required for protein synthesis, the enzyme binds to bulge-helix-bulge motifs of RNA containing a noncleaved and a cleaved splice site, the enzyme the dimer recognizes cooperatively a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line cleavage reaction Archaeoglobus fulgidus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.16 dimer structure of the enzyme-BHB RNA complex, PDB code 2A9L, the dimer recognizes cooperatively a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line cleavage reaction Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
4.6.1.16 RNA splicing endonuclease
-
 Archaeoglobus fulgidus
4.6.1.16 splicing endonuclease
-
 Archaeoglobus fulgidus