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Literature summary extracted from

  • Nakazawa, Y.; Sato, H.; Uchino, M.; Takano, K.
    Purification, characterization and cloning of phospholipase D from peanut seeds (2006), Protein J., 25, 212-223.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4
-
 Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.072
-
 phosphatidylcholine in presence of 10 mM CaCl2, 4 mM deoxycholate Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 91880
-
 calculated from sequence, PLD2 Arachis hypogaea
3.1.4.4 92000
-
 x * 92000, PLD2, SDS-PAGE Arachis hypogaea

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Arachis hypogaea
-
 L. cv. Handate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4
-
 Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 seed
-
 Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 193.7
-
-
 Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O
-
 Arachis hypogaea 1,2-diacylglycerophosphate + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 92000, PLD2, SDS-PAGE Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 PLD2
-
 Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5
-
-
 Arachis hypogaea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 4 5.5 pH 4.0: about 35% of maximal activity, pH 5.5: about 65% of maximal activity Arachis hypogaea