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Literature summary extracted from

  • Kumar, S.; Kikon, K.; Upadhyay, A.; Kanwar, S.S.; Gupta, R.
    Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3 (2005), Protein Expr. Purif., 41, 38-44.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Al3+ slight inhibition Weizmannia coagulans
3.1.1.3 Co2+ complete inhibition Weizmannia coagulans
3.1.1.3 Mn2+ complete inhibition Weizmannia coagulans
3.1.1.3 Zn2+ complete inhibition Weizmannia coagulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Weizmannia coagulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Fe3+ activates Weizmannia coagulans
3.1.1.3 Hg2+ highly activating Weizmannia coagulans
3.1.1.3 K+ activates Weizmannia coagulans
3.1.1.3 Mg2+ activates Weizmannia coagulans
3.1.1.3 additional information no effect by Na+ Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 31000
-
 x * 31000, SDS-PAGE Weizmannia coagulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Weizmannia coagulans
-
-
-
3.1.1.3 Weizmannia coagulans BTS-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 40fold to homogeneity by ammonium sulfate fractionation and ion exchange chromatography Weizmannia coagulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 additional information optimization of culture conditions, overview, optimal enzyme production at 55°C, pH 8.5 on mustard oil as carbon source and peptone/yeast extract, ratio 1:1, as nitrogen sources Weizmannia coagulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
-
 Weizmannia coagulans
3.1.1.3 4.8
-
 purified enzyme Weizmannia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Weizmannia coagulans 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Weizmannia coagulans BTS-3 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O
-
 Weizmannia coagulans 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl caprylate + H2O
-
 Weizmannia coagulans BTS-3 4-nitrophenol + caprylate
-
 ?
3.1.1.3 4-nitrophenyl formate + H2O
-
 Weizmannia coagulans 4-nitrophenol + formate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Weizmannia coagulans 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Weizmannia coagulans BTS-3 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Weizmannia coagulans 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O best substrate Weizmannia coagulans BTS-3 4-nitrophenol + palmitate
-
 ?
3.1.1.3 additional information substrate specificity, the enzyme shows preference for triacylglyceride substrates with long fatty acid chains Weizmannia coagulans ?
-
 ?
3.1.1.3 additional information substrate specificity, the enzyme shows preference for triacylglyceride substrates with long fatty acid chains Weizmannia coagulans BTS-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 31000, SDS-PAGE Weizmannia coagulans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 alkaline lipase
-
 Weizmannia coagulans
3.1.1.3 lipase
-
 Weizmannia coagulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 55
-
-
 Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 55
-
 half-life is 2 h Weizmannia coagulans
3.1.1.3 60
-
 half-life is 30 min Weizmannia coagulans
3.1.1.3 70
-
 purified enzyme, 75% remaining activity after 20 min Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
-
 Weizmannia coagulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7 9.3
-
 Weizmannia coagulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 7 10.5 purified enzyme, stable Weizmannia coagulans