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Literature summary extracted from

  • Cadel, S.; Piesse, C.; Gouzy-Darmon, C.; Cohen, P.; Foulon, T.
    Arginyl aminopeptidase (2004), Proteases in Biology and Disease (Hooper, N. M. ; Lendeckel, U. ) Springer, 2, 113-126.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 DNA and amino acid sequence determination and analysis, the gene encoding the enzyme is localized on chromosome 1, genetic organization and structure, overview Mus musculus
3.4.11.6 gene rnpep, DNA and amino acid sequence determination and analysis, localization on chromosome 1q32 in a high transcript density region, genetic organization and structure, overview Homo sapiens
3.4.11.6 testis enzyme, DNA and amino acid sequence determination and analysis, genetic organization and structure, overview, expression in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline reversible by Zn2+ Rattus norvegicus
3.4.11.6 arphamenine A
-
 Rattus norvegicus
3.4.11.6 Arphamenine B
-
 Rattus norvegicus
3.4.11.6 bestatin
-
 Rattus norvegicus
3.4.11.6 DTT
-
 Rattus norvegicus
3.4.11.6 EDTA
-
 Rattus norvegicus
3.4.11.6 EGTA
-
 Rattus norvegicus
3.4.11.6 additional information no inhibition by PMSF, pepstatin, and aprotinin Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 acrosome of germinal cells, proacrosmic granule of round spermatids Rattus norvegicus
-
-
3.4.11.6 extracellular
-
 Mus musculus
-
-
3.4.11.6 extracellular
-
 Bos taurus
-
-
3.4.11.6 extracellular the enzyme is secreted Homo sapiens
-
-
3.4.11.6 extracellular the enzyme is secreted, in PC-12 cells the active enzyme is associated to the external surface of the plassma mamabrane Rattus norvegicus
-
-
3.4.11.6 extracellular matrix
-
 Mus musculus 31012
-
3.4.11.6 extracellular matrix
-
 Homo sapiens 31012
-
3.4.11.6 extracellular matrix
-
 Rattus norvegicus 31012
-
3.4.11.6 extracellular matrix
-
 Bos taurus 31012
-
3.4.11.6 Golgi apparatus of germinal cells Rattus norvegicus 5794
-
3.4.11.6 secretory vesicle from Jurkat lymphocytes Homo sapiens 99503
-
3.4.11.6 secretory vesicle from pituitary gland, associated to membrane Bos taurus 99503
-
3.4.11.6 secretory vesicle synaptic-like vesicles in brain Rattus norvegicus 99503
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Cl- activates at physiological concentrations around 150 mM, dependent on the target peptide Rattus norvegicus
3.4.11.6 Zn2+ dependent on, zinc-metallopeptidase, the enzyme contains the binding motif HEXXHX18E Mus musculus
3.4.11.6 Zn2+ dependent on, zinc-metallopeptidase, the enzyme contains the binding motif HEXXHX18E Homo sapiens
3.4.11.6 Zn2+ dependent on, zinc-metallopeptidase, the enzyme contains the binding motif HEXXHX18E Rattus norvegicus
3.4.11.6 Zn2+ dependent on, zinc-metallopeptidase, the enzyme contains the binding motif HEXXHX18E Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 72000
-
 1 * 72000, testis enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.6 additional information Mus musculus the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development ?
-
 ?
3.4.11.6 additional information Homo sapiens the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development ?
-
 ?
3.4.11.6 additional information Bos taurus the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development ?
-
 ?
3.4.11.6 additional information Rattus norvegicus the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development, the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Bos taurus
-
-
-
3.4.11.6 Homo sapiens
-
 gene rnpep
-
3.4.11.6 Mus musculus
-
-
-
3.4.11.6 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.6 no glycoprotein
-
 Mus musculus
3.4.11.6 no glycoprotein
-
 Homo sapiens
3.4.11.6 no glycoprotein
-
 Rattus norvegicus
3.4.11.6 no glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 native enzyme from testis Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.6 release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys exopeptidase, no endoprotease activity, the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with substrates possessing Pro at the P1 position Rattus norvegicus
a
3.4.11.6 release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys exopeptidase, the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with substrate possessing Pro at the P1 position Mus musculus
a
3.4.11.6 release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys exopeptidase, the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with substrate possessing Pro at the P1 position Bos taurus
a
3.4.11.6 release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys exopeptidase, the enzyme is strictly specific for the removal of N-terminal basic residues from peptides and proteins, no activity with substrates possessing Pro at the P1 position Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.6 BE(2)-M17 cell neuroblastoma cell line Homo sapiens
-
3.4.11.6 brain highest enzyme activity Rattus norvegicus
-
3.4.11.6 Caco-2 cell colorectal adenocarcinoma cell line Homo sapiens
-
3.4.11.6 DAUDI cell B-cell line Homo sapiens
-
3.4.11.6 digestive tract high enzyme activity Homo sapiens
-
3.4.11.6 embryo
-
 Homo sapiens
-
3.4.11.6 epididymis
-
 Rattus norvegicus
-
3.4.11.6 germ cell
-
 Rattus norvegicus
-
3.4.11.6 GH3 cell pituitary tumor-derived cell line Rattus norvegicus
-
3.4.11.6 heart
-
 Rattus norvegicus
-
3.4.11.6 HeLa cell
-
 Homo sapiens
-
3.4.11.6 HL-60 cell neutrophilic promyelocyte cell line Homo sapiens
-
3.4.11.6 JURKAT cell lymphoma cell line Homo sapiens
-
3.4.11.6 K-562 cell chronic myeloid leukemia cell line Homo sapiens
-
3.4.11.6 kidney
-
 Rattus norvegicus
-
3.4.11.6 kidney high enzyme activity Homo sapiens
-
3.4.11.6 large intestine
-
 Rattus norvegicus
-
3.4.11.6 leukocyte peripheral, high enzyme activity Homo sapiens
-
3.4.11.6 Leydig cell
-
 Rattus norvegicus
-
3.4.11.6 liver
-
 Rattus norvegicus
-
3.4.11.6 liver high enzyme activity Homo sapiens
-
3.4.11.6 lung
-
 Rattus norvegicus
-
3.4.11.6 lung highest enzyme activity Homo sapiens
-
3.4.11.6 MOLT-4 cell T-cell line Homo sapiens
-
3.4.11.6 additional information ubiquitous tissue distribution, expression level is tissue- and cell-type-dependent Homo sapiens
-
3.4.11.6 additional information wide tissue distribution, expression level is tissue- and cell-type-dependent Rattus norvegicus
-
3.4.11.6 Mrc5 cell lung cell line Homo sapiens
-
3.4.11.6 muscle
-
 Rattus norvegicus
-
3.4.11.6 pancreas high enzyme activity Homo sapiens
-
3.4.11.6 pancreas high enzyme activity Rattus norvegicus
-
3.4.11.6 PC-12 cell adrenal pheochromocytoma cell line Rattus norvegicus
-
3.4.11.6 peritubular cell somatic, testis Rattus norvegicus
-
3.4.11.6 pituitary gland intermediate lobe Bos taurus
-
3.4.11.6 placenta high enzyme activity Homo sapiens
-
3.4.11.6 RAJI cell lymphoblast-like cell line Homo sapiens
-
3.4.11.6 salivary gland high enzyme activity Homo sapiens
-
3.4.11.6 Sertoli cell
-
 Rattus norvegicus
-
3.4.11.6 small intestine
-
 Rattus norvegicus
-
3.4.11.6 spermatid late Rattus norvegicus
-
3.4.11.6 spleen high enzyme activity Homo sapiens
-
3.4.11.6 spleen high enzyme activity Rattus norvegicus
-
3.4.11.6 stomach high enzyme activity Rattus norvegicus
-
3.4.11.6 T-lymphocyte
-
 Homo sapiens
-
3.4.11.6 testis highest enzyme activity, mainly expressed in late spermatids during maturation Rattus norvegicus
-
3.4.11.6 uterus high enzyme activity Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Arg-beta-atrial natriuretic factor1-20 + H2O
-
 Rattus norvegicus Arg + beta-atrial natriuretic factor1-20
-
 ?
3.4.11.6 Arg-Lys-somatostatin-14 + 2 H2O sequential removal of basic N-terminal residues Rattus norvegicus Arg + Lys + somatostatin-14
-
 ?
3.4.11.6 Arg-Met-enkephalin + H2O
-
 Rattus norvegicus Arg + Met-enkephalin
-
 ?
3.4.11.6 Arg-neurokinin A + H2O
-
 Rattus norvegicus Arg + neurokinin A
-
 ?
3.4.11.6 kallidin-10 + H2O
-
 Rattus norvegicus Lys + bradykinin
-
 ?
3.4.11.6 leukotriene A4 + H2O weak in vitro activity Rattus norvegicus L-Arg + leukotriene B4
-
 ?
3.4.11.6 additional information the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development Mus musculus ?
-
 ?
3.4.11.6 additional information the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development Homo sapiens ?
-
 ?
3.4.11.6 additional information the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development Bos taurus ?
-
 ?
3.4.11.6 additional information the enzyme probably is involved in the final stages of peptide and protein precursor processing and maturation mechanisms via the exopeptidase pathway and thereby in some inflammatory processes and tumour development, the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides Rattus norvegicus ?
-
 ?
3.4.11.6 additional information the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides, no activity with bradykinin, neurotensin precursor and substance P, no activity with substrate possessing Pro at the P1 position Rattus norvegicus ?
-
 ?
3.4.11.6 additional information the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides, no activity with substrate possessing Pro at the P1 position Mus musculus ?
-
 ?
3.4.11.6 additional information the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides, no activity with substrate possessing Pro at the P1 position Homo sapiens ?
-
 ?
3.4.11.6 additional information the enzyme from testis preferably removes Arg and/or Lys residues from the N-terminus of various peptides, no activity with substrate possessing Pro at the P1 position Bos taurus ?
-
 ?
3.4.11.6 thymopentin + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 monomer 1 * 72000, testis enzyme Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 Ap-B
-
 Mus musculus
3.4.11.6 Ap-B
-
 Homo sapiens
3.4.11.6 Ap-B
-
 Rattus norvegicus
3.4.11.6 Ap-B
-
 Bos taurus
3.4.11.6 arginyl aminopeptidase
-
 Mus musculus
3.4.11.6 arginyl aminopeptidase
-
 Homo sapiens
3.4.11.6 arginyl aminopeptidase
-
 Rattus norvegicus
3.4.11.6 arginyl aminopeptidase
-
 Bos taurus
3.4.11.6 arylamidase
-
 Rattus norvegicus
3.4.11.6 Cl--activated arinine aminopeptidase
-
 Rattus norvegicus
3.4.11.6 cytosol aminopeptidase IV
-
 Rattus norvegicus
3.4.11.6 More the enzyme belongs to the M1 peptidase family Mus musculus
3.4.11.6 More the enzyme belongs to the M1 peptidase family Homo sapiens
3.4.11.6 More the enzyme belongs to the M1 peptidase family Rattus norvegicus
3.4.11.6 More the enzyme belongs to the M1 peptidase family Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.6 additional information
-
 broad activity range Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.6 Rattus norvegicus
-
-
 4.9