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Literature summary extracted from

  • Tocchini-Valentini, G.D.; Fruscoloni, P.; Tocchini-Valentini, G.P.
    Coevolution of tRNA intron motifs and tRNA endonuclease architecture in archaea (2005), Proc. Natl. Acad. Sci. USA, 102, 15418-15422.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.16 gene ARCFU, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus
4.6.1.16 gene METJA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Methanocaldococcus jannaschii
4.6.1.16 gene SULSO, DNA and amino acid sequence determination and analysis, coexpression of His-tagged SULSO alpha-subunit and untagged beta-subunit in Escherichia coli strain BL21(DE3) Saccharolobus solfataricus
4.6.1.16 gene XENLA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Xenopus laevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.16 Mg2+
-
Xenopus laevis
4.6.1.16 Mg2+
-
Saccharolobus solfataricus
4.6.1.16 Mg2+
-
Methanocaldococcus jannaschii
4.6.1.16 Mg2+
-
Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.16 Archaeoglobus fulgidus
-
gene ARCFU
-
4.6.1.16 Methanocaldococcus jannaschii
-
gene METJA
-
4.6.1.16 Saccharolobus solfataricus
-
gene SULSO
-
4.6.1.16 Xenopus laevis
-
gene XENLA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.16 copurification of recombinant His-tagged SULSO alpha-subunit and untagged beta-subunit from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Saccharolobus solfataricus
4.6.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Xenopus laevis
4.6.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Methanocaldococcus jannaschii
4.6.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.16 additional information the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs and bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview Saccharolobus solfataricus ?
-
?
4.6.1.16 additional information the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview Xenopus laevis ?
-
?
4.6.1.16 additional information the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview Methanocaldococcus jannaschii ?
-
?
4.6.1.16 additional information the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview Archaeoglobus fulgidus ?
-
?

Subunits

EC Number Subunits Comment Organism
4.6.1.16 dimer homodimer, alpha'2 enzyme architecture, structure overview Archaeoglobus fulgidus
4.6.1.16 tetramer heterotetramer, alpha2beta2 enzyme architecture, structure overview Saccharolobus solfataricus
4.6.1.16 tetramer homotetramer, alpha4 enzyme architecture, structure overview Xenopus laevis
4.6.1.16 tetramer homotetramer, alpha4 enzyme architecture, structure overview Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.6.1.16 tRNA endonuclease
-
Xenopus laevis
4.6.1.16 tRNA endonuclease
-
Saccharolobus solfataricus
4.6.1.16 tRNA endonuclease
-
Methanocaldococcus jannaschii
4.6.1.16 tRNA endonuclease
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.16 22
-
assay at Xenopus laevis
4.6.1.16 65
-
assay at Saccharolobus solfataricus
4.6.1.16 65
-
assay at Methanocaldococcus jannaschii
4.6.1.16 65
-
assay at Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.16 7.5
-
assay at Xenopus laevis
4.6.1.16 7.5
-
assay at Saccharolobus solfataricus
4.6.1.16 7.5
-
assay at Methanocaldococcus jannaschii
4.6.1.16 7.5
-
assay at Archaeoglobus fulgidus