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Literature summary extracted from

  • Krstanovic, M.; Brgles, M.; Halassy, B.; Frkanec, R.; Vrdoljak, A.; Branovic, K.; Tomasic, J.; Benedetti, F.
    Purification and characterization of L,(L/D)-aminopeptidase from guinea pig serum (2006), Prep. Biochem. Biotechnol., 36, 175-195.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 amastatin strong inhibition Cavia porcellus
3.4.11.2 bestatin strong inhibition Cavia porcellus
3.4.11.2 metal chelators strong inhibition Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.6
-
 L-Ala-4-nitroanilide
-
 Cavia porcellus
3.4.11.2 18
-
 L-Ala-D-iso-Gln-meso-DAP(omegaNH2)-D-Ala-D-Ala
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 extracellular
-
 Cavia porcellus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 175000
-
 gel filtration Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 native L,(L/D)-aminopeptidase 700fold from serum to homogeneity in 4 chromatographic steps Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 serum
-
 Cavia porcellus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 enkephalin + H2O enkephalins comprising L,L- and L,D-peptide bonds Cavia porcellus ?
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Cavia porcellus L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-D-iso-Gln-meso-DAP(omegaNH2)-D-Ala-D-Ala + H2O
-
 Cavia porcellus L-Ala + D-iso-Gln-meso-DAP(omegaNH2)-D-Ala-D-Ala product identification and stereochemic determination ?
3.4.11.2 additional information the enzyme hydrolyzes several peptides with N-terminal L-amino acids, but not with N-terminal D-amino acids, no activity with L-Asp-L-Asp Cavia porcellus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 L,(L/D)-aminopeptidase
-
 Cavia porcellus