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Literature summary extracted from

  • Munoz, A.; Raso, M.J.; Pineda, M.; Piedras, P.
    Degradation of ureidoglycolate in French bean (Phaseolus vulgaris) is catalysed by a ubiquitous ureidoglycolate urea-lyase (2006), Planta, 224, 175-184.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.2.3 L-asparagine
-
 Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.3 EDTA 10 mM, complete inhibition, activity can be restored by Mn2+ and Co2+, slightly by Zn2+ Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.3 3.9
-
 ureidoglycolate 50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.2.3 Co2+
-
 Phaseolus vulgaris
4.3.2.3 Mg2+
-
 Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.3 38000
-
 6 * 38000, SDS-PAGE Phaseolus vulgaris
4.3.2.3 240000
-
 SDS-PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.3 ureidoglycolate Phaseolus vulgaris
-
 glyoxylate + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.3 Phaseolus vulgaris
-
 L. cv. Great Northern
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.3 ammonium sulfate precipitation, gel filtration, heat treatment, hydrophobic interaction chromatography, immunoaffinity chromatography Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.3 additional information all tissues Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.2.3 4.439
-
 50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.3 ureidoglycolate
-
 Phaseolus vulgaris glyoxylate + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.3 hexamer 6 * 38000, SDS-PAGE Phaseolus vulgaris

Synonyms

EC Number Synonyms Comment Organism
4.3.2.3 ureidoglycolate urea-lyase
-
 Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.2.3 50 60
-
 Phaseolus vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.3 0.018
-
 ureidoglycolate 50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.3 7.8
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.2.3 6 9
-
 Phaseolus vulgaris