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Literature summary extracted from

  • Garcia, N.A.T.; Iribarne, C.; Lopez, M.; Herrera-Cervera, J.A.; Lluch, C.
    Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and characterization (2005), Plant Physiol. Biochem., 43, 355-361.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 EDTA inhibits the acid trehalase by 70% Phaseolus vulgaris
3.2.1.28 Fe3+ inhibits the acid trehalase by 50% Phaseolus vulgaris
3.2.1.28 Hg+ inhibits the acid trehalase by 95% Phaseolus vulgaris
3.2.1.28 validamycin A inhibits the acid trehalase Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.109
-
 alpha,alpha-trehalose pH 6.3, 37°C Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Co2+ activates the acid trehalase Phaseolus vulgaris
3.2.1.28 K+ activates the acid trehalase Phaseolus vulgaris
3.2.1.28 Li+ activates the acid trehalase Phaseolus vulgaris
3.2.1.28 Mg2+ activates the acid trehalase by about 30% Phaseolus vulgaris
3.2.1.28 Mn2+ activates the acid trehalase by about 30% Phaseolus vulgaris
3.2.1.28 Na+ activates the acid trehalase by about 30% Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 42000
-
 x * 42000 + x * 45000, SDS-PAGE Phaseolus vulgaris
3.2.1.28 45000
-
 x * 42000 + x * 45000, SDS-PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Phaseolus vulgaris
-
 2 D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 acid trehalase 425fold from root nodules, by ammonium sulfate fractionation, ion exchange chromatography, gel filtration, and native PAGE Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 root nodule
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 175.7
-
 purified acid trehalase Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Phaseolus vulgaris 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O preferred substrate of acid trehalase Phaseolus vulgaris 2 D-glucose
-
 ?
3.2.1.28 cellobiose + H2O
-
 Phaseolus vulgaris 2 D-glucose
-
 ?
3.2.1.28 maltose + H2O
-
 Phaseolus vulgaris 2 D-glucose
-
 ?
3.2.1.28 melibiose + H2O
-
 Phaseolus vulgaris D-glucose + D-galactose
-
 ?
3.2.1.28 additional information no activity of acid trehalase with lactose and turanose Phaseolus vulgaris ?
-
 ?
3.2.1.28 raffinose + H2O
-
 Phaseolus vulgaris ?
-
 ?
3.2.1.28 sucrose + H2O
-
 Phaseolus vulgaris D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 42000 + x * 45000, SDS-PAGE Phaseolus vulgaris

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 acid trehalase
-
 Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 37
-
 assay at Phaseolus vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 25 75
-
 Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 40
-
 30 min, stable up to Phaseolus vulgaris
3.2.1.28 45 50 loss of 40% activity, purified acid trehalase Phaseolus vulgaris
3.2.1.28 60
-
 nearly complete inactivation of purified acid trehalase Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 3.9
-
 acid trehalase Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 3.6 6.6
-
 Phaseolus vulgaris