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Literature summary extracted from

  • Kanno, T.; Komatsu, A.; Kasai, K.; Dubouzet, J.G.; Sakurai, M.; Ikejiri-Kanno, Y.; Wakasa, K.; Tozawa, Y.
    Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway (2005), Plant Physiol., 138, 2260-2268.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.27 expression in Saccharomyces cerevisiae Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.27 A369L Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 A380S mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
4.1.3.27 A532Y mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 G518A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 G521A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 G522A increased sensitivity to Trp feedback inhibition of anthranilate synthase alpha-subunit OASA2 mutant Oryza sativa
4.1.3.27 G522Y increased sensitivity to Trp feedback inhibition of anthranilate synthase alpha-subunit OASA2 mutant Oryza sativa
4.1.3.27 L520F mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
4.1.3.27 L530D mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
4.1.3.27 N351A increased sensitivity to Trp feedback inhibition of anthranilate synthase alpha-subunit OASA2 mutant Oryza sativa
4.1.3.27 N351D mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
4.1.3.27 N363A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 N363D mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 P364A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 P364L mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 P366A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
4.1.3.27 S126A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 S356A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 Y349A mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
4.1.3.27 Y349F mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
4.1.3.27 Y367A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 Y367A/L530D accumulation of free Trp in cells expressing the mutant OASA2 is 2.3fold of that of cells expressing wild-type D126F /L530D Oryza sativa
4.1.3.27 Y516A nonfunctional protein, anthranilate synthase alpha-subunit OASA2 Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-Trp feedback-inhibition, IC50: 0.0063 mM for wild-type enzyme, 0.0933 mM for mutant S126F, 0.0316 mM for mutant Y367A, 0.0749 mM for mutant A369L, 0.101 mM for mutant Y367A/L530D and 0.0583 mM for mutant A369L/L530D Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.039
-
chorismate pH 8.3, 32°C, A369L/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.043
-
chorismate pH 8.3, 32°C, A369L mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.043
-
chorismate pH 8.3, 32°C, wild-type enzyme, anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.049
-
chorismate pH 8.3, 32°C, S126F mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.058
-
chorismate pH 8.3, 32°C, Y367A/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.062
-
chorismate pH 8.3, 32°C, S126F/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.076
-
chorismate pH 8.3, 32°C, Y367A mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + NH4+
-
Oryza sativa anthranilate + pyruvate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.27 OASA2 anthranilate synthase alpha-subunit Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.27 0.092
-
chorismate pH 8.3, 32°C, Y367A mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.108
-
chorismate pH 8.3, 32°C, S126F mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.22
-
chorismate pH 8.3, 32°C, A369L mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.225
-
chorismate pH 8.3, 32°C, wild-type enzyme, anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.405
-
chorismate pH 8.3, 32°C, A369L/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.49
-
chorismate pH 8.3, 32°C, Y367A/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 0.573
-
chorismate pH 8.3, 32°C, S126F/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
4.1.3.27 6.08
-
chorismate pH 8.3, 32°C, S126F/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.3.27 0.0063
-
feedback-inhibition, IC50: 0.0063 mM for wild-type enzyme, 0.0933 mM for mutant S126F, 0.0316 mM for mutant Y367A, 0.0749 mM for mutant A369L, 0.101 mM for mutant Y367A/L530D and 0.0583 mM for mutant A369L/L530D Oryza sativa L-Trp