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Literature summary extracted from

  • Korennykh, A.V.; Piccirilli, J.A.; Correll, C.C.
    The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxins (2006), Nat. Struct. Mol. Biol., 13, 436-443.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.23 mutants K63D, R21D/K28D and R21D/K28D/K63D Aspergillus restrictus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.23 K63D mutant with decreased activity for sarcin/ricin loop-cleavage of ribosomes Aspergillus restrictus
4.6.1.23 R21D/K28D mutant with decreased activity for sarcin/ricin loop-cleavage of ribosomes Aspergillus restrictus
4.6.1.23 R21D/K28D/K63D mutant with decreased activity for sarcin/ricin loop-cleavage of ribosomes Aspergillus restrictus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.23 0.076
-
 SRL sarcin/ricin loop in ribosomal RNA, 10 mM Tris-HCl, 0-5 mM KCl, pH 7-7.4, 37°C Aspergillus restrictus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.23 KCl at low salt concentrations less than 5 mM maximum of cleavage rate Aspergillus restrictus
4.6.1.23 KCl at low salt concentrations of less than 5 mM the enzyme has its maximum cleavage rate Aspergillus restrictus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.23 Aspergillus restrictus
-
 alpha-sarcin-like ribotoxin restrictocin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.23 C-25 SP ion-exchange column, pH 5.5, 4°C Aspergillus restrictus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.23 SRL + H2O sarcin/ricin loop in ribosomal RNA Aspergillus restrictus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.23 alpha-sarcin-like ribotoxin restrictocin
-
 Aspergillus restrictus