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Literature summary extracted from
- Pseudomonas aeruginosa elastase and elastolysis revisited: recent developments (1991), Mol. Microbiol., 5, 2315-2321.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.26 | expression in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.26 | ammonium chloride | extracellular elastase activity decreases if cells are cultured in the presence of ammonium chloride | Pseudomonas aeruginosa |  |
| 3.4.24.26 | D-glucose | extracellular elastase activity decreases if cells are cultured in the presence of glucose | Pseudomonas aeruginosa | |
| 3.4.24.26 | EDTA | - |
Pseudomonas aeruginosa | |
| 3.4.24.26 | additional information | extracellular elastase activity decreases if cells are cultured in the presence of sub-inhibitory concentrations of certain antibiotics | Pseudomonas aeruginosa | |
| 3.4.24.26 | o-phenanthroline | - |
Pseudomonas aeruginosa | |
| 3.4.24.26 | phosphoramidon | - |
Pseudomonas aeruginosa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.26 | cytoplasm | synthesis of the precursor preproelastase | Pseudomonas aeruginosa | 5737 | - |
| 3.4.24.26 | extracellular | - |
Pseudomonas aeruginosa | - |
- |
| 3.4.24.26 | extracellular | mature elastase | Pseudomonas aeruginosa | - |
- |
| 3.4.24.26 | periplasm | proelastase I and II | Pseudomonas aeruginosa | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.26 | Zinc | located at the active site | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 33000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
| 3.4.24.26 | 33500 | - |
proelastase II, SDS-PAGE | Pseudomonas aeruginosa |
| 3.4.24.26 | 50000 | - |
proelastase I, SDS-PAGE | Pseudomonas aeruginosa |
| 3.4.24.26 | 53600 | - |
preproelastase (precursor),SDS-PAGE | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.26 | Pseudomonas aeruginosa | P14756 | - |
- |
| 3.4.24.26 | Pseudomonas aeruginosa | P14756 | exhibits greatly reduced amounts of elastolytic activity | - |
| 3.4.24.26 | Pseudomonas aeruginosa PAO-E64 | P14756 | exhibits greatly reduced amounts of elastolytic activity | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.26 | carbobenzoxy-Gly-Leu-NH2 + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | carbobenzoxy-Gly-Phe-NH2 + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | carbobenzoxy-Gly-Tyr-NH2 + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | casein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | casein + H2O | - |
Pseudomonas aeruginosa PAO-E64 | ? | - |
? | |
| 3.4.24.26 | Elastin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | Elastin + H2O | - |
Pseudomonas aeruginosa PAO-E64 | ? | - |
? | |
| 3.4.24.26 | Fibrin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | Fibrin + H2O | - |
Pseudomonas aeruginosa PAO-E64 | ? | - |
? | |
| 3.4.24.26 | human alpha-1 proteinase inhibitor + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | human collagen + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | human gamma-interferon + H2O | - |
Pseudomonas aeruginosa | fragments of human gamma-interferon | - |
? | |
| 3.4.24.26 | immunoglobulin A + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | immunoglobulin A + H2O | - |
Pseudomonas aeruginosa PAO-E64 | ? | - |
? | |
| 3.4.24.26 | immunoglobulin G + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | Laminin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | Laminin + H2O | - |
Pseudomonas aeruginosa PAO-E64 | ? | - |
? | |
| 3.4.24.26 | secretory immunoglobulin A + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.26 | ? | x * 33000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.26 | Pseudomonas aeruginosa elastase | - |
Pseudomonas aeruginosa |
| 3.4.24.26 | Pseudomonas elastase | - |
Pseudomonas aeruginosa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 32 | - |
the enzyme is less active at temperatures above 32°C | Pseudomonas aeruginosa |
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