Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Basten, D.E.J.W.; Moers, A.P.H.A.; van Ooyen, A.J.J.; Schaap, P.J.
    Characterisation of Aspergillus niger prolyl aminopeptidase (2005), Mol. Gen. Genet., 272, 673-679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.5 expressed in Escherichia coli Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 PCMB
-
 Aspergillus niger
3.4.11.5 TLCK
-
 Aspergillus niger
3.4.11.5 TPCK
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.5 0.037
-
 Pro-4-nitroanilide
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 50000
-
 6 * 50000, SDS-PAGE Aspergillus niger
3.4.11.5 316000
-
 gel filtration of the native enzyme Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Aspergillus niger Q96VT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.5 four chromatography steps Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.5 0.21
-
 Pro-4-nitroanilide, cell free extract at 30°C, pH 7.5 Aspergillus niger
3.4.11.5 6.6
-
 PLSRTLSVAAKK, at 30°C, pH 7.5 Aspergillus niger
3.4.11.5 33.4
-
 Pro-Leu-Gly-NH2, at 30°C, pH 7.5 Aspergillus niger
3.4.11.5 55.5
-
 PPGFSPFR, at 30°C, pH 7.5 Aspergillus niger
3.4.11.5 85.5
-
 Pro-Ala, at 30°C, pH 7.5 Aspergillus niger
3.4.11.5 290
-
 Pro-4-nitroanilide, four-fold purified enzyme at 30°C, pH 7.5 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 Ala-Pro + H2O
-
 Aspergillus niger L-alanine + L-proline
-
 ?
3.4.11.5 L-hydroxyprolyl-2-naphthylamide + H2O
-
 Aspergillus niger L-hydroxyproline + 2-naphthylamine
-
 ?
3.4.11.5 PLSRTLSVAAKK + H2O
-
 Aspergillus niger LSRTLSVAAKK + L-proline
-
 ?
3.4.11.5 PPGFSPFR + H2O
-
 Aspergillus niger PGFSPFR + L-proline
-
 ?
3.4.11.5 Pro-2-naphthylamide + H2O
-
 Aspergillus niger 2-naphthylamine + L-proline
-
 ?
3.4.11.5 Pro-4-nitroanilide + H2O
-
 Aspergillus niger 4-nitroaniline + L-proline
-
 ?
3.4.11.5 Pro-Ala + H2O
-
 Aspergillus niger L-alanine + L-proline
-
 ?
3.4.11.5 Pro-Leu-Gly-NH2 + H2O
-
 Aspergillus niger Leu-Gly-NH2 + L-proline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.5 hexamer 6 * 50000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.4.11.5 PAP
-
 Aspergillus niger
3.4.11.5 Prolyl aminopeptidase
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.5
-
 37 significant activity decrease at 50°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 7.5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.5 7 8
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.5 5 8 highest stability found between pH 6 an 7 Aspergillus niger