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Literature summary extracted from

  • Garrido, M.N.; Lisa, T.A.; Albelo, S.; Lucchesi, G.I.; Domenech, C.E.
    Identification of the Pseudomonas aeruginosa acid phosphatase as an phosphorylcholine phosphatase activity (1990), Mol. Cell. Biochem., 94, 89-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 additional information the enzyme is induced by betaine, choline, and N,N-dimethylglycine as sole carbon and nitrogen sources Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 phosphorylcholine substrate inhibition Pseudomonas aeruginosa
3.1.3.2 Phosphorylethanolamine substrate inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 additional information
-
 additional information kinetics Pseudomonas aeruginosa
3.1.3.2 0.2
-
 phosphorylcholine pH 5.0, 37°C Pseudomonas aeruginosa
3.1.3.2 0.7
-
 Phosphorylethanolamine pH 5.0, 37°C Pseudomonas aeruginosa
3.1.3.2 2
-
 4-nitrophenyl phosphate pH 5.0, 37°C Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 periplasm
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Mg2+
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 partially by ammonium sulfate fractionation and gel filtration Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 additional information the enzyme is induced by betaine, choline, and N,N-dimethylglycine as sole carbon and nitrogen sources Pseudomonas aeruginosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
 ?
3.1.3.2 additional information the enzyme shows high substrate specificity, no activity with pyridoxal 5'-phosphate, glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-diphosphate, AMP, ADP, and ATP Pseudomonas aeruginosa ?
-
 ?
3.1.3.2 phosphorylcholine + H2O preferred substrate compared to phosphorylethanolamine Pseudomonas aeruginosa choline + phosphate
-
 ?
3.1.3.2 phosphorylethanolamine + H2O
-
 Pseudomonas aeruginosa ethanolamine + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
 assay at Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 additional information
-
 additional information inhibition kinetics Pseudomonas aeruginosa
3.1.3.2 1
-
 phosphorylcholine pH 5.0, 37°C Pseudomonas aeruginosa
3.1.3.2 3
-
 Phosphorylethanolamine pH 5.0, 37°C Pseudomonas aeruginosa