BRENDA - Enzyme Database

A highly active alkaline phosphatase from the marine bacterium cobetia

Yu Plisova, E.; Balabanova, L.A.; Ivanova, E.P.; Kozhemyako, V.B.; Mikhailov, V.V.; Agafonova, E.V.; Rasskazov, V.A.; Mar. Biotechnol. 7, 173-178 (2005)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.1
2-mercaptoethanol
10 mM, 70% decrease in activity
Cobetia marina
3.1.3.1
Cd2+
5 mM, 30% inhibition
Cobetia marina
3.1.3.1
Co2+
5 mM, 60% inhibition
Cobetia marina
3.1.3.1
Cu2+
5 mM, 40% inhibition
Cobetia marina
3.1.3.1
dithiothreitol
10 mM, 95% decrease in activity
Cobetia marina
3.1.3.1
glycerol
concentrations of 10%, 20% and 40% inhibit the activity by 35%, 60%, and 90%
Cobetia marina
3.1.3.1
additional information
iodoacetamide and NaN3 at 2-10 mM concentration have no effect on activity
Cobetia marina
3.1.3.1
Ni2+
5 mM, 27% inhibition
Cobetia marina
3.1.3.1
p-chloromercuribenzoate
0.1-0.2 mM, 85%-100% inhibition
Cobetia marina
3.1.3.1
Zn2+
5 mM, complete inhibition
Cobetia marina
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.1
K+
0.1 M, stimulates
Cobetia marina
3.1.3.1
Na+
0.1 M, stimulates
Cobetia marina
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.1
55000
-
1 * 55000, SDS-PAGE
Cobetia marina
3.1.3.1
62000
-
gel filtration
Cobetia marina
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.1
Cobetia marina
-
-
-
3.1.3.1
Cobetia marina KMM MC-296
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.1
-
Cobetia marina
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
2',3-AMP + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
5'-AMP + H2O
90% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-AMP + H2O
90% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-CMP + H2O
80% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
cytidine + phosphate
-
-
-
?
3.1.3.1
5'-dCMP + H2O
80% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
deoxycytidine + phosphate
-
-
-
?
3.1.3.1
5'-GMP + H2O
40% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
guanosine + phosphate
-
-
-
?
3.1.3.1
5'-UMP + H2O
30% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
uridine + phosphate
-
-
-
?
3.1.3.1
5'-UMP + H2O
30% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
uridine + phosphate
-
-
-
?
3.1.3.1
ADP + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
ADP + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
?
-
-
-
?
3.1.3.1
alpha-glycerophosphate + H2O
60% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
glycerol + phosphate
-
-
-
?
3.1.3.1
ATP + H2O
10% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
ATP + H2O
10% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
?
-
-
-
?
3.1.3.1
beta-glycerophosphate + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
glycerol + phosphate
-
-
-
?
3.1.3.1
beta-glycerophosphate + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
glycerol + phosphate
-
-
-
?
3.1.3.1
NADP+ + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
p-nitrophenyl phosphate + H2O
-
666229
Cobetia marina
p-nitrophenol + phosphate
-
-
-
?
3.1.3.1
UDP-glucose + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.1
monomer
1 * 55000, SDS-PAGE
Cobetia marina
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.1
50
-
stable up to, 15 min
Cobetia marina
3.1.3.1
55
-
15 min, about 20% loss of activity
Cobetia marina
3.1.3.1
58
-
15 min, about 55% loss of activity
Cobetia marina
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.1
10.3
-
-
Cobetia marina
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.1
9.9
10.7
about 50% of maximal activity at pH 9.9 and pH 10.7
Cobetia marina
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.3.1
5
-
15 min, 20% loss of activity
Cobetia marina
3.1.3.1
6
-
15 min, 10% loss of activity
Cobetia marina
3.1.3.1
7
11
15 min, stable
Cobetia marina
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.3.1
Cobetia marina
chromatofocusing
-
4.5
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.1
2-mercaptoethanol
10 mM, 70% decrease in activity
Cobetia marina
3.1.3.1
Cd2+
5 mM, 30% inhibition
Cobetia marina
3.1.3.1
Co2+
5 mM, 60% inhibition
Cobetia marina
3.1.3.1
Cu2+
5 mM, 40% inhibition
Cobetia marina
3.1.3.1
dithiothreitol
10 mM, 95% decrease in activity
Cobetia marina
3.1.3.1
glycerol
concentrations of 10%, 20% and 40% inhibit the activity by 35%, 60%, and 90%
Cobetia marina
3.1.3.1
additional information
iodoacetamide and NaN3 at 2-10 mM concentration have no effect on activity
Cobetia marina
3.1.3.1
Ni2+
5 mM, 27% inhibition
Cobetia marina
3.1.3.1
p-chloromercuribenzoate
0.1-0.2 mM, 85%-100% inhibition
Cobetia marina
3.1.3.1
Zn2+
5 mM, complete inhibition
Cobetia marina
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.1
K+
0.1 M, stimulates
Cobetia marina
3.1.3.1
Na+
0.1 M, stimulates
Cobetia marina
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.1
55000
-
1 * 55000, SDS-PAGE
Cobetia marina
3.1.3.1
62000
-
gel filtration
Cobetia marina
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.1
-
Cobetia marina
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
2',3-AMP + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
5'-AMP + H2O
90% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-AMP + H2O
90% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-CMP + H2O
80% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
cytidine + phosphate
-
-
-
?
3.1.3.1
5'-dCMP + H2O
80% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
deoxycytidine + phosphate
-
-
-
?
3.1.3.1
5'-GMP + H2O
40% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
guanosine + phosphate
-
-
-
?
3.1.3.1
5'-UMP + H2O
30% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
uridine + phosphate
-
-
-
?
3.1.3.1
5'-UMP + H2O
30% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
uridine + phosphate
-
-
-
?
3.1.3.1
ADP + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
ADP + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
?
-
-
-
?
3.1.3.1
alpha-glycerophosphate + H2O
60% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
glycerol + phosphate
-
-
-
?
3.1.3.1
ATP + H2O
10% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
ATP + H2O
10% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
?
-
-
-
?
3.1.3.1
beta-glycerophosphate + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
glycerol + phosphate
-
-
-
?
3.1.3.1
beta-glycerophosphate + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina KMM MC-296
glycerol + phosphate
-
-
-
?
3.1.3.1
NADP+ + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
3.1.3.1
p-nitrophenyl phosphate + H2O
-
666229
Cobetia marina
p-nitrophenol + phosphate
-
-
-
?
3.1.3.1
UDP-glucose + H2O
20% of activity with p-nitrophenyl phosphate
666229
Cobetia marina
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.1
monomer
1 * 55000, SDS-PAGE
Cobetia marina
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.1
50
-
stable up to, 15 min
Cobetia marina
3.1.3.1
55
-
15 min, about 20% loss of activity
Cobetia marina
3.1.3.1
58
-
15 min, about 55% loss of activity
Cobetia marina
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.1
10.3
-
-
Cobetia marina
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.1
9.9
10.7
about 50% of maximal activity at pH 9.9 and pH 10.7
Cobetia marina
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.3.1
5
-
15 min, 20% loss of activity
Cobetia marina
3.1.3.1
6
-
15 min, 10% loss of activity
Cobetia marina
3.1.3.1
7
11
15 min, stable
Cobetia marina
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.3.1
Cobetia marina
chromatofocusing
-
4.5