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Literature summary extracted from

  • Nampoothiri, K.M.; Nagy, V.; Kovacs, K.; Szakacs, G.; Pandey, A.
    L-Leucine aminopeptidase production by filamentous Aspergillus fungi (2005), Lett. Appl. Microbiol., 41, 498-504.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 Al3+ 98% inhibition at 2 mM, complete inhibition at 10 mM Aspergillus oryzae
3.4.11.1 Al3+ strong inhibition Aspergillus sojae
3.4.11.1 Ca2+ 70% inhibition at 2 mM Aspergillus oryzae
3.4.11.1 Ca2+
-
 Aspergillus sojae
3.4.11.1 Cd2+ 80% inhibition at 2 mM Aspergillus oryzae
3.4.11.1 Cd2+
-
 Aspergillus sojae
3.4.11.1 Co2+ 39% activation at 2 mM, 40% inhibition at 10 mM Aspergillus oryzae
3.4.11.1 Co2+ activates at 2 mM, inhibits at 10 mM and above Aspergillus sojae
3.4.11.1 Cu2+ 86% inhibition at 2 mM, complete inhibition at 10 mM Aspergillus oryzae
3.4.11.1 Cu2+ strong inhibition Aspergillus sojae
3.4.11.1 Fe2+ 15% activation at 2 mM, 55% inhibition at 10 mM Aspergillus oryzae
3.4.11.1 Fe2+
-
 Aspergillus sojae
3.4.11.1 Mg2+ 50% inhibition at 2 mM Aspergillus oryzae
3.4.11.1 Mg2+
-
 Aspergillus sojae
3.4.11.1 Mn2+ 60% inhibition at 2 mM Aspergillus oryzae
3.4.11.1 Mn2+
-
 Aspergillus sojae
3.4.11.1 additional information inhibition of enzymes from strains NRRL 6271 and NRRL 1988 by different metal ions at different concentrations, overview Aspergillus sojae
3.4.11.1 Zn2+ 26% inhibition at 2 mM, 86% at 10 mM, and 95% at 20 mM Aspergillus oryzae
3.4.11.1 Zn2+
-
 Aspergillus sojae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ 39% activation at 2 mM, 40% inhibition at 10 mM Aspergillus oryzae
3.4.11.1 Co2+ activates at 2 mM, inhibits at 10 mM and above Aspergillus sojae
3.4.11.1 Fe2+ 15% activation at 2 mM, 55% inhibition at 10 mM Aspergillus oryzae
3.4.11.1 K+ activates at 10-20 mM Aspergillus sojae
3.4.11.1 additional information no effect by K+ and Na+ Aspergillus oryzae
3.4.11.1 Na+ activates at 10-20 mM Aspergillus sojae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Aspergillus oryzae
-
 other strains show low enzyme activity
-
3.4.11.1 Aspergillus oryzae NRRL 6270
-
 other strains show low enzyme activity
-
3.4.11.1 Aspergillus sojae
-
 strains NRRL 1988 and NRRL 6271, other strains show low enzyme activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 high LAP production in strain NRRL 6270, solid-state fermentation, process optimization, overview Aspergillus oryzae
3.4.11.1 high LAP production in strains NRRL 1988 and NRRL 6271, solid-state fermentation, process optimization, overview Aspergillus sojae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 additional information optimization of filamentous culture conditions Aspergillus oryzae
-
3.4.11.1 additional information optimization of filamentous culture conditions Aspergillus sojae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 additional information
-
-
 Aspergillus oryzae
3.4.11.1 additional information
-
-
 Aspergillus sojae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 L-leucine aminopeptidase
-
 Aspergillus oryzae
3.4.11.1 L-leucine aminopeptidase
-
 Aspergillus sojae
3.4.11.1 LAP
-
 Aspergillus oryzae
3.4.11.1 LAP
-
 Aspergillus sojae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 65 70 at pH 8.5 Aspergillus oryzae
3.4.11.1 65 70 strain NRRL 6271, at pH 8.5 Aspergillus sojae
3.4.11.1 75
-
 strain NRRL 1988, at pH 8.5 Aspergillus sojae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
 at 50°C Aspergillus oryzae
3.4.11.1 8.5 9 at 50°C Aspergillus sojae