EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.68 | expression in Escherichia coli | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.68 | Gu-HCl | 500 mM reduces cleavage to 60%, 1 M reduces cleavage to 0% | Saccharomyces cerevisiae | |
3.4.22.68 | additional information | no inhibitory effects are observed with Triton X100 (1 M), imidazole (300 mM), reduced glutathione (20 mM), maltose (20 mM), glycerol (20% v/v), ethylene glycol (20% v/v), sucrose (20% w/v), ethanol (10% v/v) | Saccharomyces cerevisiae | |
3.4.22.68 | NaCl | 500 mM reduces cleavage to 60%, 1 M reduces cleavage to 30% | Saccharomyces cerevisiae | |
3.4.22.68 | Urea | 2 M reduces cleavage to 95%, 3 M reduces cleavage to 5% | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.68 | 31000 | - |
x * 31000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.68 | Saccharomyces cerevisiae | Q02724 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.68 | - |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.22.68 | -70°C, 50% glycerol, 75 mM Tris-HCl pH 8.0, 0.5 mM DTT, 1 mM EDTA, 6 months, no detectable decay of activity | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.68 | small ubiquitin-like modifier protein + H2O | - |
Saccharomyces cerevisiae | ? | Ulp1 catalyzes the proteolytic processing of SUMO to its mature form | ? | |
3.4.22.68 | SUMO-GFP fusion substrate + H2O | pH 8.0, 25°C, in the presence of 5 mM 2-mercaptoethanol | Saccharomyces cerevisiae | SUMO + GFP | - |
? | |
3.4.22.68 | SUMO-MMP13 + H2O | cleavage occurs only to 60% | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.68 | ? | x * 31000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.68 | SUMO protease 1 | - |
Saccharomyces cerevisiae |
3.4.22.68 | Ulp1 | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.68 | 22 | 37 | - |
Saccharomyces cerevisiae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.68 | 4 | 37 | - |
Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.68 | 8 | 9 | - |
Saccharomyces cerevisiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.68 | 6 | 10 | - |
Saccharomyces cerevisiae |