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Literature summary extracted from

  • Kwon, A.R.; Trame, C.B.; McKay, D.B.
    Kinetics of protein substrate degradation by HslUV (2004), J. Struct. Biol., 146, 141-147.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.25.2
-
 Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.25.2
-
 Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.25.2 0.001
-
 SulA mutant P8L pH 5.5, 37°C, recombinant enzyme Haemophilus influenzae
3.4.25.2 0.0023
-
 SulA pH 5.5, 37°C, wild-type SulA, recombinant enzyme Haemophilus influenzae
3.4.25.2 0.0032
-
 SulA mutant I37V pH 5.5, 37°C, recombinant enzyme Haemophilus influenzae
3.4.25.2 0.0034
-
 SulA mutant F10A pH 5.5, 37°C, recombinant enzyme Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.25.2 KCl
-
 Haemophilus influenzae
3.4.25.2 Mg2+
-
 Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.25.2 SulA + H2O Haemophilus influenzae specific substrate degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.25.2 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.25.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.25.2 additional information the enzyme degrades only the SulA moiety of recombinant fusion proteins, the fused proteins, e.g. the green fluorescent protein, are not hydrolyzed Haemophilus influenzae ?
-
 ?
3.4.25.2 SulA + H2O specific substrate degradation Haemophilus influenzae ?
-
 ?
3.4.25.2 SulA + H2O specific substrate degradation, activities with SulA mutant protein substrates F10A, I37V, and P8L Haemophilus influenzae ?
-
 ?
3.4.25.2 SulA mutant F10A + H2O
-
 Haemophilus influenzae ?
-
 ?
3.4.25.2 SulA mutant I37V + H2O
-
 Haemophilus influenzae ?
-
 ?
3.4.25.2 SulA mutant P8L + H2O
-
 Haemophilus influenzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.25.2 hexamer the HslU12HslV12 protease-chaperone complex Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
3.4.25.2 HslUV
-
 Haemophilus influenzae
3.4.25.2 HslUV protease-chaperone complex
-
 Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.25.2 30
-
 assay at Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.25.2 0.033
-
 SulA mutant P8L pH 5.5, 37°C, recombinant enzyme Haemophilus influenzae
3.4.25.2 0.05
-
 SulA pH 5.5, 37°C, wild-type SulA, recombinant enzyme Haemophilus influenzae
3.4.25.2 0.063
-
 SulA mutant F10A pH 5.5, 37°C, recombinant enzyme Haemophilus influenzae
3.4.25.2 0.068
-
 SulA mutant I37V pH 5.5, 37°C, recombinant enzyme Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.25.2 7.8
-
 assay at Haemophilus influenzae

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.25.2 ATP dependent on Haemophilus influenzae