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Literature summary extracted from

  • Ketkar, A.D.; Shenoy, A.R.; Ramagopal, U.A.; Visweswariah, S.S.; Suguna, K.
    A Structural Basis for the Role of Nucleotide Specifying Residues in Regulating the Oligomerization of the Rv1625c Adenylyl Cyclase from M. tuberculosis (2006), J. Mol. Biol., 356, 904-916.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.1 triple mutant K296E/F363R/D365C, 2.65 A resolution Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.1 F363R mutant with poor adenylate cyclase acitivity Mycobacterium tuberculosis
4.6.1.1 K296E mutant with about 400fold reduced enzyme activity Mycobacterium tuberculosis
4.6.1.1 K296E/D365C double mutant with severely compromised enzyme activity Mycobacterium tuberculosis
4.6.1.1 K296E/F363R/D365C triple mutant with severely compromised enzyme activity Mycobacterium tuberculosis
4.6.1.1 additional information from Ala232 N-terminal truncated mutant has much lower enzyme activity compared with the wild type, significant reduction in the apparent Vmax with no appreciable change in the apparent K' for ATP Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.1 Mn2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.1 29000
-
 2 * 29000, wild type, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Mycobacterium tuberculosis P9WQ35
-
-
4.6.1.1 Mycobacterium tuberculosis H37Rv P9WQ35
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Mycobacterium tuberculosis cAMP + diphosphate
-
 ?
4.6.1.1 ATP
-
 Mycobacterium tuberculosis H37Rv cAMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.1 dimer 2 * 29000, wild type, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 adenylyl cyclase
-
 Mycobacterium tuberculosis
4.6.1.1 Rv1625c
-
 Mycobacterium tuberculosis