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Literature summary extracted from

  • Rodriguez, A.; Wall, L.; Raptis, S.; Zarkadas, C.G.; Meighen, E.
    Different sites for fatty acid activation and acyl transfer by the synthetase subunit of fatty acid reductase: acylation of a cysteinyl residue (1988), Biochim. Biophys. Acta, 964, 266-275.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.50 34000
-
 x * 57000 + x * 34000 + x * 52000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE Photobacterium phosphoreum
1.2.1.50 52000
-
 x * 57000 + x * 34000 + x * 52000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE Photobacterium phosphoreum
1.2.1.50 57000
-
 x * 57000 + x * 34000 + x * 52000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE Photobacterium phosphoreum
6.2.1.19 52000
-
 calculation from amino acid composition Photobacterium phosphoreum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.50 tetradecanoyl-CoA + NADPH + H+ Photobacterium phosphoreum
-
 tetradecanal + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.50 Photobacterium phosphoreum
-
-
-
6.2.1.19 Photobacterium phosphoreum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.50
-
 Photobacterium phosphoreum

Storage Stability

EC Number Storage Stability Organism
1.2.1.50 -20°C, 50 mM phosphate buffer, pH 7.0, 0.2 M NaCl, 50 mM beta-mercaptoethanol, 30% glycerol, several months Photobacterium phosphoreum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.50 tetradecanoyl-CoA + NADPH + H+
-
 Photobacterium phosphoreum tetradecanal + CoA + NADP+
-
 ?
6.2.1.19 ATP + myristic acid + protein
-
 Photobacterium phosphoreum AMP + diphosphate + myristoyl-protein thiolester
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.50 polymer x * 57000 + x * 34000 + x * 52000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE Photobacterium phosphoreum