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Literature summary extracted from

  • Liu, F.; Tachibana, S.; Taira, T.; Ishihara, M.; Kato, F.; Yasuda, M.
    Purification and characterization of a high molecular mass serine carboxypeptidase from Monascus pilosus (2004), J. Ind. Microbiol. Biotechnol., 31, 572-580.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.5 1,2-epoxy-3-(p-nitrophenoxy)propane 1 mM, 14% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 2,2'-dipyridyl 1 mM, 21% inhibition, isoenzyme MpiCP-1; 1 mM, 22% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 2-mercaptoethanol 1 mM, 13% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 chymostatin 1 mM, 36% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 diisopropylfluorophosphate 1 mM, 39% inhibition, isoenzyme MpiCP-1; 1 mM, 67% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 E-64 1 mM, 10% inhibition, isoenzyme MpiCP-2; 1 mM, 22% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 EDTA 1 mM, 38% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 Elastatinal 1 mM, 46% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 leupeptin 1 mM, 24% inhibition, isoenzyme MpiCP-1; 1 mM, 60% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 Monoiodoacetic acid 1 mM, 14% inhibition; 1 mM, 25% inhibition Monascus pilosus
3.4.16.5 additional information no inhibition with 1 mM EDTA or chymostatin Monascus pilosus
3.4.16.5 Nalpha-tosyl-L-Lys-chloromethyl ketone 1 mM, 13% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 Nalpha-tosyl-L-Phe-chloromethyl ketone 1 mM, 12% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 PCMB 1 mM, 28% inhibition, isoenzyme MpiCP-1; 1 mM, 97% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 pepstatin A 1 mM, 11% inhibition, isoenzyme MpiCP-2; 1 mM, 19% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 Phenylmethylsulfonylfluoride 1 mM, 61% inhibition, isoenzyme MpiCP-1; 1 mM, complete inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 phosphoramidon 1 mM, 17% inhibition, isoenzyme MpiCP-1; 1 mM, 41% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 piperastatin A 1 mM, 17% inhibition, isoenzyme MpiCP-2; 1 mM, 87% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 tosylphenylalanylchloromethane 1 mM, 35% inhibition, isoenzyme MpiCP-1 Monascus pilosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.5 1.33
-
 benzyloxycarbonyl-Tyr-Glu pH 4.0, 37°C, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 1.55
-
 benzyloxycarbonyl-Tyr-Glu pH 3.5, 37°C, isoenzyme MpiCP-2 Monascus pilosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.16.5 CaCl2 1 mM, 18% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 CdCl2 1 mM, 18% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 CoCl2 1 mM, 23% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 CuCl 1 mM, 33% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 CuCl 1 mM, 84% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 CuCl2 1 mM, 36% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 CuCl2 1 mM, 73% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 FeCl2 1 mM, 35% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 FeCl2 1 mM, 73% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 FeCl3 1 mM, 37% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 FeCl3 1 mM, 86% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 HgCl2 1 mM, complete inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 MgCl2 1 mM, 15% inhibition, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 NiCl2 1 mM, 12% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 PbCl2 1 mM, 47% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 SnCl2 1 mM, 47% inhibition, isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 ZnSO4 1 mM, 46% inhibition, isoenzyme MpiCP-2 Monascus pilosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.16.5 59000
-
 38 * 59000, isoenzyme MpiCP-2, SDS-PAGE Monascus pilosus
3.4.16.5 61000
-
 2 * 61000, isoenzyme MpiCP-1, SDS-PAGE Monascus pilosus
3.4.16.5 125000
-
 isoenzyme MpiCP-1, gel filtration Monascus pilosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.5 Monascus pilosus
-
 isoenzyme MpiCP-1
-
3.4.16.5 Monascus pilosus
-
 isoenzyme MpiCP-2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.16.5 glycoprotein 11% carbohydrate, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 glycoprotein 33% carbohydrate, isoenzyme MpiCP-2 Monascus pilosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.5 isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 isoenzyme MpiCP-2 Monascus pilosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.16.5 3.91
-
-
 Monascus pilosus
3.4.16.5 4
-
 isoenzyme MpiCP-2 Monascus pilosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.5 benzoyl-Gly-Arg + H2O isoenzyme MpiCP-2 Monascus pilosus benzoyl-Gly + Arg
-
 ?
3.4.16.5 benzoyl-Gly-Lys + H2O isoenzyme MpiCP-2 Monascus pilosus benzoyl-Gly + Lys
-
 ?
3.4.16.5 benzyloxycarbonyl-Ala-Glu + H2O isoenzyme MpiCP-1 Monascus pilosus benzyloxycarbonyl-Ala + Glu
-
 ?
3.4.16.5 benzyloxycarbonyl-Ala-Glu + H2O isoenzyme MpiCP-2 Monascus pilosus benzyloxycarbonyl-Ala + Glu
-
 ?
3.4.16.5 benzyloxycarbonyl-Glu-Phe + H2O isoenzyme MpiCP-1 Monascus pilosus benzyloxycarbonyl-Glu + Phe
-
 ?
3.4.16.5 Benzyloxycarbonyl-Glu-Tyr + H2O isoenzyme MpiCP-1 Monascus pilosus Benzyloxycarbonyl-Glu + Tyr
-
 ?
3.4.16.5 Benzyloxycarbonyl-Glu-Tyr + H2O isoenzyme MpiCP-2 Monascus pilosus Benzyloxycarbonyl-Glu + Tyr
-
 ?
3.4.16.5 benzyloxycarbonyl-Phe-Ala + H2O isoenzyme MpiCP-1 Monascus pilosus benzyloxycarbonyl-Phe + Ala
-
 ?
3.4.16.5 benzyloxycarbonyl-Phe-Ala + H2O isoenzyme MpiCP-2 Monascus pilosus benzyloxycarbonyl-Phe + Ala
-
 ?
3.4.16.5 benzyloxycarbonyl-Phe-Gly + H2O isoenzyme MpiCP-2 Monascus pilosus benzyloxycarbonyl-Phe + Gly
-
 ?
3.4.16.5 benzyloxycarbonyl-Phe-Leu + H2O isoenzyme MpiCP-1 Monascus pilosus benzyloxycarbonyl-Phe + Leu
-
 ?
3.4.16.5 benzyloxycarbonyl-Phe-Leu + H2O isoenzyme MpiCP-2 Monascus pilosus benzyloxycarbonyl-Phe + Leu
-
 ?
3.4.16.5 benzyloxycarbonyl-Pro-Leu-Gly + H2O isoenzyme MpiCP-2 Monascus pilosus benzyloxycarbonyl-Pro-Leu + Gly
-
 ?
3.4.16.5 benzyloxycarbonyl-Tyr-Glu + H2O preferred substrate for isoenzyme MpiCP-1 Monascus pilosus benzyloxycarbonyl-Tyr + Glu
-
 ?
3.4.16.5 benzyloxycarbonyl-Tyr-Glu + H2O preferred substrate for isoenzyme MpiCP-2 Monascus pilosus benzyloxycarbonyl-Tyr + Glu
-
 ?
3.4.16.5 additional information no activity with benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Gly-Pro, benzyloxycarbonyl-Gly-Pro-Leu, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, benzoyl-Gly-Lys, Leu-Gly-Gly Monascus pilosus ?
-
 ?
3.4.16.5 additional information no activity with benzyloxycarbonylGlu-Phe, benzyloxycarbonyl-Gly-Leu, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Gly-Pro, benzyloxycarbonyl-Gly-Pro-Leu, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, Leu-Gly-Gly Monascus pilosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.16.5 dimer 2 * 61000, isoenzyme MpiCP-1, SDS-PAGE Monascus pilosus
3.4.16.5 oligomer 38 * 59000, isoenzyme MpiCP-2, SDS-PAGE Monascus pilosus

Synonyms

EC Number Synonyms Comment Organism
3.4.16.5 MpiCP-1
-
 Monascus pilosus
3.4.16.5 MpiCP-2
-
 Monascus pilosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.16.5 50
-
 isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 50
-
 isoenzyme MpiCP-2 Monascus pilosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.16.5 50
-
 pH 6.0, 15 min, isoenzyme MpiCP-2 is stable up to Monascus pilosus
3.4.16.5 55
-
 pH 6.0, 15 min, isoenzyme MpiCP-1 is stable Monascus pilosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.16.5 723
-
 benzyloxycarbonyl-Tyr-Glu pH 4.0, 37°C, isoenzyme MpiCP-1 Monascus pilosus
3.4.16.5 2039
-
 benzyloxycarbonyl-Tyr-Glu pH 3.5, 37°C, isoenzyme MpiCP-2 Monascus pilosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.5 3.5
-
 isoenzyme MpiCP-2 Monascus pilosus
3.4.16.5 4
-
 isoenzyme MpiCP-1 Monascus pilosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.16.5 2 8 37°C, 1 h, isoenzyme MpiCP-1 is stable Monascus pilosus
3.4.16.5 5.5 6.6 37°C, 1 h, isoenzyme MpiCP-2 is stable up to Monascus pilosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.16.5 Monascus pilosus isoenzyme MpiCP-1
-
 3.7
3.4.16.5 Monascus pilosus isoenzyme MpiCP-2
-
 4