Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sopina, V.A.
    Acid metallophosphatase from the ameba Amoeba proteus (2004), J. Evol. Biochem. Physiol., 40, 28-36.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 1,10-phenanthroline reversible Amoeba proteus
3.1.3.2 EDTA reversible Amoeba proteus
3.1.3.2 EGTA only partly reversible Amoeba proteus
3.1.3.2 Fe2+ complete inhibition at 5-50 mM FeSO4 or Fe2(SO4)3 Amoeba proteus
3.1.3.2 Fe3+ slight inhibition Amoeba proteus
3.1.3.2 additional information the enzyme is tartrate-resistant Amoeba proteus
3.1.3.2 Zn2+ inhibits 2 of 3 isozymes Amoeba proteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ca2+ activates all 3 isozymes Amoeba proteus
3.1.3.2 Mg2+ activates all 3 isozymes Amoeba proteus
3.1.3.2 additional information no effect by Li+ Amoeba proteus
3.1.3.2 Zn2+ the enzyme is a Zn-metallophosphatase containing an essential zinc ion in the active site Amoeba proteus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Amoeba proteus
-
 3 isozymes
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.2 a phosphate monoester + H2O = an alcohol + phosphate the enzyme is a Zn-metallophosphatase containing an essential zinc ion in the active site Amoeba proteus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 0.203
-
 substrate 1-naphthyl phosphate Amoeba proteus
3.1.3.2 2.712
-
 substrate 4-nitrophenyl phosphate Amoeba proteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Amoeba proteus 1-naphthol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Amoeba proteus 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 tartrate-resistant acid phosphatase
-
 Amoeba proteus
3.1.3.2 TR-AcPh
-
 Amoeba proteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 30
-
 assay at Amoeba proteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4
-
 assay at Amoeba proteus