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Literature summary extracted from

  • Suzuki, H.; Sawai, Y.; Suzuki, T.; Kawai, K.
    Purification and characterization of an extracellular alpha-neoagarooligosaccharide hydrolase from Bacillus sp. MK03 (2002), J. Biosci. Bioeng., 93, 456-463.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.159 AgNO3 2 mM, 9% residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 CuCl2 2 mM, 7% residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 EDTA 2 mM, 47% residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 HgCl2 2 mM, no residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 NiCl2 2 mM, 10% residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 p-chloromercuribenzoate 0.2 mM 60% residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 SDS 2 mM, 53% residual activity Bacillus sp. (in: Bacteria)
3.2.1.159 SrCl2 2 mM, 43% residual activity Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.159 MgCl2 2 mM, 163% of initial activity Bacillus sp. (in: Bacteria)
3.2.1.159 SnCl2 2 mM, 121% of initial activity Bacillus sp. (in: Bacteria)
3.2.1.159 ZnCl2 2 mM, 128% of initial activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.159 42000
-
8 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.2.1.159 320000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.159 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.159 Bacillus sp. (in: Bacteria) MK03
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.159 culture fluid
-
Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.159 22.2
-
pH 7.0, 30°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.159 alpha-1,3-neoagarobiose + H2O 40% hydrolysis within 30 min Bacillus sp. (in: Bacteria) 3,6-anhydro-L-galactose + D-galactose
-
?
3.2.1.159 alpha-1,3-neoagarobiose + H2O 40% hydrolysis within 30 min Bacillus sp. (in: Bacteria) MK03 3,6-anhydro-L-galactose + D-galactose
-
?
3.2.1.159 neoagarohexaose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) 3,6-anhydro-L-galactose + agaropentaose
-
?
3.2.1.159 neoagarohexaose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) MK03 3,6-anhydro-L-galactose + agaropentaose
-
?
3.2.1.159 neoagarotetraose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) 3,6-anhydro-L-galactose + agarotriose
-
?
3.2.1.159 neoagarotetraose + H2O complete hydrolysis within 30 min Bacillus sp. (in: Bacteria) MK03 3,6-anhydro-L-galactose + agarotriose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.159 More N-terminal amino acid sequence Bacillus sp. (in: Bacteria)
3.2.1.159 octamer 8 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.159 30
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.159 30
-
stable up to, 30 min, 96% residual activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.159 6.1
-
-
Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.159 6.8 8.6 stable within this range Bacillus sp. (in: Bacteria)