EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.13 | Triton X-100 | 0.3%, about 50% activation | Streptomyces sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.13 | 2-mercaptoethanol | 1 mM, 94% residual activity | Streptomyces sp. | |
3.1.1.13 | Ag+ | 1 mM, 75% residual activity | Streptomyces sp. | |
3.1.1.13 | deoxycholic acid | 1 mM, 60% inhibition | Streptomyces sp. | |
3.1.1.13 | dithiothreitol | 1 mM, 0% residual activity | Streptomyces sp. | |
3.1.1.13 | Hg2+ | 1 mM, 50% residual activity | Streptomyces sp. | |
3.1.1.13 | additional information | not inhibitory: phenylmethylsulfonylfluoride | Streptomyces sp. | |
3.1.1.13 | p-chloromercuribenzoate | 1 mM, 78% residual activity | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.13 | 0.09 | - |
cholesteryl linoleate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 0.34 | - |
cholesteryl myristate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 1.1 | - |
p-nitrophenyl myristate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 2.1 | - |
p-nitrophenyl palmitate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 4.3 | - |
p-nitrophenyl laurate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 6.8 | - |
cholesteryl palmitate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 7 | - |
cholesteryl stearate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 7 | - |
cholesteryl acetate | pH 7.0, 37°C | Streptomyces sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.13 | 23600 | - |
x * 23600, SDS-PAGE | Streptomyces sp. |
3.1.1.13 | 163000 | - |
gel filtration | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.13 | Streptomyces sp. | - |
- |
- |
3.1.1.13 | Streptomyces sp. X9 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.13 | - |
Streptomyces sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.13 | culture fluid | - |
Streptomyces sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.13 | 0.01 | - |
substrate p-nitrophenyl acetate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 0.67 | - |
substrate p-nitrophenyl laurate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 2.08 | - |
substrate p-nitrophenyl palmitate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 4.97 | - |
substrate p-nitrophenyl myristate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 11.8 | - |
substrate cholesteryl acetate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 13.4 | - |
substrate cholesteryl palmitate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 13.4 | - |
substrate cholesteryl stearate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 41 | - |
substrate cholesteryl myristate, pH 7.0, 37°C | Streptomyces sp. |
3.1.1.13 | 78.8 | - |
substrate cholesteryl linoleate, pH 7.0, 37°C | Streptomyces sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.13 | stable during long-term aqueous storage at room temperature | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.13 | cholesteryl acetate + H2O | - |
Streptomyces sp. | cholesterol + acetic acid | - |
? | |
3.1.1.13 | cholesteryl linoleate + H2O | - |
Streptomyces sp. | cholesterol + linoleic acid | - |
? | |
3.1.1.13 | cholesteryl linoleate + H2O | - |
Streptomyces sp. X9 | cholesterol + linoleic acid | - |
? | |
3.1.1.13 | cholesteryl myristate + H2O | - |
Streptomyces sp. | cholesterol + myristic acid | - |
? | |
3.1.1.13 | cholesteryl myristate + H2O | - |
Streptomyces sp. X9 | cholesterol + myristic acid | - |
? | |
3.1.1.13 | cholesteryl palmitate + H2O | - |
Streptomyces sp. | cholesterol + palmitic acid | - |
? | |
3.1.1.13 | cholesteryl palmitate + H2O | - |
Streptomyces sp. X9 | cholesterol + palmitic acid | - |
? | |
3.1.1.13 | cholesteryl stearate + H2O | - |
Streptomyces sp. | cholesterol + stearic acid | - |
? | |
3.1.1.13 | cholesteryl stearate + H2O | - |
Streptomyces sp. X9 | cholesterol + stearic acid | - |
? | |
3.1.1.13 | p-nitrophenyl acetate + H2O | - |
Streptomyces sp. | p-nitrophenol + acetate | - |
? | |
3.1.1.13 | p-nitrophenyl acetate + H2O | - |
Streptomyces sp. X9 | p-nitrophenol + acetate | - |
? | |
3.1.1.13 | p-nitrophenyl laurate + H2O | - |
Streptomyces sp. | p-nitrophenol + laurate | - |
? | |
3.1.1.13 | p-nitrophenyl myristate + H2O | - |
Streptomyces sp. | p-nitrophenol + myristic acid | - |
? | |
3.1.1.13 | p-nitrophenyl palmitate + H2O | - |
Streptomyces sp. | p-nitrophenol + palmitic acid | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.13 | ? | x * 23600, SDS-PAGE | Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.13 | 40 | - |
- |
Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.13 | 70 | - |
30 min, 60% residual activity | Streptomyces sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.13 | 1.4 | - |
p-nitrophenyl laurate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 2.9 | - |
p-nitrophenyl palmitate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 4.5 | - |
p-nitrophenyl myristate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 23.5 | - |
cholesteryl myristate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 31.2 | - |
cholesteryl linoleate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 70.8 | - |
cholesteryl stearate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 96.7 | - |
cholesteryl palmitate | pH 7.0, 37°C | Streptomyces sp. | |
3.1.1.13 | 110 | - |
cholesteryl acetate | pH 7.0, 37°C | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.13 | 6 | - |
- |
Streptomyces sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.13 | 5 | 7.5 | - |
Streptomyces sp. |