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Literature summary extracted from

  • Xiang, H.; Takaya, N.; Hoshino, T.
    Novel cholesterol esterase secreted by Streptomyces persists during aqueous long-term storage (2006), J. Biosci. Bioeng., 101, 19-25.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 Triton X-100 0.3%, about 50% activation Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 2-mercaptoethanol 1 mM, 94% residual activity Streptomyces sp.
3.1.1.13 Ag+ 1 mM, 75% residual activity Streptomyces sp.
3.1.1.13 deoxycholic acid 1 mM, 60% inhibition Streptomyces sp.
3.1.1.13 dithiothreitol 1 mM, 0% residual activity Streptomyces sp.
3.1.1.13 Hg2+ 1 mM, 50% residual activity Streptomyces sp.
3.1.1.13 additional information not inhibitory: phenylmethylsulfonylfluoride Streptomyces sp.
3.1.1.13 p-chloromercuribenzoate 1 mM, 78% residual activity Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.13 0.09
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 0.34
-
cholesteryl myristate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 1.1
-
p-nitrophenyl myristate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 2.1
-
p-nitrophenyl palmitate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 4.3
-
p-nitrophenyl laurate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 6.8
-
cholesteryl palmitate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 7
-
cholesteryl stearate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 7
-
cholesteryl acetate pH 7.0, 37°C Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 23600
-
x * 23600, SDS-PAGE Streptomyces sp.
3.1.1.13 163000
-
gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Streptomyces sp.
-
-
-
3.1.1.13 Streptomyces sp. X9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.13
-
Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 culture fluid
-
Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 0.01
-
substrate p-nitrophenyl acetate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 0.67
-
substrate p-nitrophenyl laurate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 2.08
-
substrate p-nitrophenyl palmitate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 4.97
-
substrate p-nitrophenyl myristate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 11.8
-
substrate cholesteryl acetate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 13.4
-
substrate cholesteryl palmitate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 13.4
-
substrate cholesteryl stearate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 41
-
substrate cholesteryl myristate, pH 7.0, 37°C Streptomyces sp.
3.1.1.13 78.8
-
substrate cholesteryl linoleate, pH 7.0, 37°C Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
3.1.1.13 stable during long-term aqueous storage at room temperature Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl acetate + H2O
-
Streptomyces sp. cholesterol + acetic acid
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces sp. cholesterol + linoleic acid
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Streptomyces sp. X9 cholesterol + linoleic acid
-
?
3.1.1.13 cholesteryl myristate + H2O
-
Streptomyces sp. cholesterol + myristic acid
-
?
3.1.1.13 cholesteryl myristate + H2O
-
Streptomyces sp. X9 cholesterol + myristic acid
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces sp. cholesterol + palmitic acid
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Streptomyces sp. X9 cholesterol + palmitic acid
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Streptomyces sp. cholesterol + stearic acid
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Streptomyces sp. X9 cholesterol + stearic acid
-
?
3.1.1.13 p-nitrophenyl acetate + H2O
-
Streptomyces sp. p-nitrophenol + acetate
-
?
3.1.1.13 p-nitrophenyl acetate + H2O
-
Streptomyces sp. X9 p-nitrophenol + acetate
-
?
3.1.1.13 p-nitrophenyl laurate + H2O
-
Streptomyces sp. p-nitrophenol + laurate
-
?
3.1.1.13 p-nitrophenyl myristate + H2O
-
Streptomyces sp. p-nitrophenol + myristic acid
-
?
3.1.1.13 p-nitrophenyl palmitate + H2O
-
Streptomyces sp. p-nitrophenol + palmitic acid
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 ? x * 23600, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.13 40
-
-
Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.13 70
-
30 min, 60% residual activity Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.13 1.4
-
p-nitrophenyl laurate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 2.9
-
p-nitrophenyl palmitate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 4.5
-
p-nitrophenyl myristate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 23.5
-
cholesteryl myristate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 31.2
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 70.8
-
cholesteryl stearate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 96.7
-
cholesteryl palmitate pH 7.0, 37°C Streptomyces sp.
3.1.1.13 110
-
cholesteryl acetate pH 7.0, 37°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 6
-
-
Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.13 5 7.5
-
Streptomyces sp.