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Literature summary extracted from

  • Seyit, G.; Rockel, B.; Baumeister, W.; Peters, J.
    Size matters: the spindle form of the 6 MDA tripeptidylpeptidase II from drosophila melanogaster stabilizes the activated state (2006), J. Biol. Chem., 281, 25723-25733.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.10 expression in Escherichia coli Drosophila sp. (in: flies)

General Stability

EC Number General Stability Organism
3.4.14.10 spindle formation (at TPP II concentrations above 0.03 mg/ml) by strand paring causes both significant thermodynamic and kinetic stabilization Drosophila sp. (in: flies)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.10 0.44
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 7.5, 30°C, recombinant enzyme Drosophila sp. (in: flies)
3.4.14.10 0.47
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 7.5, 30°C, native enzyme Drosophila sp. (in: flies)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.10 Drosophila sp. (in: flies)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.10
-
 Drosophila sp. (in: flies)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.10 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Drosophila sp. (in: flies) Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.10 More TPP II is a large homooligomeric complex of about 6 MDa. Spindle formation (at TPP II concentrations above 0.03 mg/ml) by strand paring causes both significant thermodynamic and kinetic stabilization Drosophila sp. (in: flies)

Synonyms

EC Number Synonyms Comment Organism
3.4.14.10 TPP II
-
 Drosophila sp. (in: flies)