EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.7 | expression of soluble C-terminally His-tagged enzyme, possessing an N-terminal human trypsin II signal peptide, in Spodoptera frugiperda Sf9 insect cells using the baculovirus transfection system | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.7 | 1,10-phenanthroline | IC50 is 0.06 mM | Homo sapiens | |
3.4.11.7 | amastatin | IC50 is 157 nM | Homo sapiens | |
3.4.11.7 | angiotensin II | competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+ | Homo sapiens | |
3.4.11.7 | angiotensin III | competitive against Glu-4-methylcoumaryl-7-amide | Homo sapiens | |
3.4.11.7 | angiotensin IV | competitive feedback inhibition, negative regulatory function in absence or presence of Ca2+; IC50 is 0.010 mM | Homo sapiens | |
3.4.11.7 | bradykinin | - |
Homo sapiens | |
3.4.11.7 | Ca2+ | inhibits activity with Gln-substrate, regulatory function and influence on substrate specificity | Homo sapiens | |
3.4.11.7 | cholecystokinin-8 | competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+ | Homo sapiens | |
3.4.11.7 | chromogranin A | competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+ | Homo sapiens | |
3.4.11.7 | E64 | slight inhibition at 1 mM | Homo sapiens | |
3.4.11.7 | kallidin | competitive against Glu-4-methylcoumaryl-7-amide | Homo sapiens | |
3.4.11.7 | leupeptin | slight inhibition at 1 mM | Homo sapiens | |
3.4.11.7 | Met-enkephalin | - |
Homo sapiens | |
3.4.11.7 | Mg2+ | inhibits the activity with Glu-substrate moderately | Homo sapiens | |
3.4.11.7 | Mn2+ | inhibits the activity with Glu-substrate moderately | Homo sapiens | |
3.4.11.7 | additional information | inhibitory effects of peptide hormones on enzyme activity in presence or absence of Ca2+, overview, no or poor inhibition by 4-(amidinophenyl)-methanesulfonyl fluoride, pepstatin, and bestatin | Homo sapiens | |
3.4.11.7 | Neurokinin A | - |
Homo sapiens | |
3.4.11.7 | neurokinin B | competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+ | Homo sapiens | |
3.4.11.7 | parathyroid hormone residues 69-84 | - |
Homo sapiens | |
3.4.11.7 | Substance P | - |
Homo sapiens | |
3.4.11.7 | Zn2+ | zinc-metallopeptidase, inhibits the activity with Glu- and Gln-substrates | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.7 | additional information | - |
additional information | kinetics and substrate specificity in presence or absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 0.4 | - |
Glu-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ | Homo sapiens | |
3.4.11.7 | 0.6 | - |
Gln-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 1.2 | - |
Glu-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 1.4 | - |
Asp-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ | Homo sapiens | |
3.4.11.7 | 2.3 | - |
Asp-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 7.1 | - |
Gln-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.7 | membrane | type II membrane-bound enzyme, membrane-spanning | Homo sapiens | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.7 | Ca2+ | activates activity with some substrates, decreases activity with other substrates, required for activity with Asp-/Glu-substrates, regulatory function and influence on substrate specificity | Homo sapiens | |
3.4.11.7 | Zn2+ | zinc-metallopeptidase, inhibits the activity with Glu- and Gln-substrates | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.7 | angiotensin II + H2O | Homo sapiens | angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in regulation of blood pressure in the brain | Asp + angiotensin III | - |
? | |
3.4.11.7 | cholecystokinin-8 + H2O | Homo sapiens | i.e. CCK8 | ? | - |
? | |
3.4.11.7 | chromogranin A + H2O | Homo sapiens | - |
? | - |
? | |
3.4.11.7 | neurokinin B + H2O | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.7 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.11.7 | glycoprotein | - |
Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.7 | recombinant soluble enzyme His-tagged from Sf9 insect cells to homogeneity by ultrafiltration, dialysis, anion exchange and cobalt affinity chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.7 | brain | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.7 | additional information | - |
- |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.7 | angiotensin II + H2O | angiotensin is the most active compound of the renin-angiotensin-system, RAS, involved in regulation of blood pressure in the brain | Homo sapiens | Asp + angiotensin III | - |
? | |
3.4.11.7 | angiotensin II + H2O | i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | Homo sapiens | Asp + angiotensin III | i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
3.4.11.7 | angiotensin III + H2O | i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, low activity in absence of Ca2+ | Homo sapiens | ? + angiotensin IV | - |
? | |
3.4.11.7 | Asp-4-methylcoumaryl-7-amide + H2O | low activity in absence and presence of Ca2+ | Homo sapiens | Asp + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.7 | cholecystokinin-8 + H2O | i.e. CCK8 | Homo sapiens | ? | - |
? | |
3.4.11.7 | cholecystokinin-8 + H2O | i.e. CCK8 or DYMGWMDF-NH2 | Homo sapiens | Asp + YMGWMDF-NH2 | - |
? | |
3.4.11.7 | chromogranin A + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.11.7 | chromogranin A + H2O | i.e. EEEEEMAVVPQGLFRG-NH2 | Homo sapiens | Glu + EEEEMAVVPQGLFRG-NH2 | - |
? | |
3.4.11.7 | Gln-4-methylcoumaryl-7-amide + H2O | low activity in absence and presence of Ca2+ | Homo sapiens | Gln + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.7 | Glu-4-methylcoumaryl-7-amide + H2O | low activity in absence of Ca2+ | Homo sapiens | Glu + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.7 | kallidin + H2O | i.e. Lys-bradykinin or KRPPGFSPFR, the reaction is performed only in absence of Ca2+ | Homo sapiens | Lys + bradykinin | i.e. RPPGFSPFR | ? | |
3.4.11.7 | additional information | substrate specificity in presence or absence of Ca2+ | Homo sapiens | ? | - |
? | |
3.4.11.7 | neurokinin B + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.11.7 | neurokinin B + H2O | i.e. DMHDFFVGLM-NH2 | Homo sapiens | Asp + MHDFFVGLM-NH2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.7 | dimer | - |
Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.7 | aminopeptidase A | - |
Homo sapiens |
3.4.11.7 | APA | - |
Homo sapiens |
3.4.11.7 | More | the enzyme belongs to the M1 family | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.7 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.7 | 2.8 | - |
Asp-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 9.5 | - |
Gln-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 12.2 | - |
Asp-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ | Homo sapiens | |
3.4.11.7 | 13.3 | - |
Glu-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in absence of Ca2+ | Homo sapiens | |
3.4.11.7 | 14.1 | - |
Gln-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ | Homo sapiens | |
3.4.11.7 | 33.3 | - |
Glu-4-methylcoumaryl-7-amide | recombinant enzyme, pH 7.5, 37°C, in presence of 1 mM Ca2+ | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.7 | 7 | - |
- |
Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.7 | additional information | - |
pH-profile, recombinant enzyme | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.4.11.7 | 0.000157 | - |
IC50 is 157 nM | Homo sapiens | amastatin | |
3.4.11.7 | 0.01 | - |
IC50 is 0.010 mM | Homo sapiens | angiotensin IV | |
3.4.11.7 | 0.024 | - |
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+ | Homo sapiens | cholecystokinin-8 | |
3.4.11.7 | 0.049 | - |
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+ | Homo sapiens | chromogranin A | |
3.4.11.7 | 0.06 | - |
competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+ | Homo sapiens | neurokinin B | |
3.4.11.7 | 0.06 | - |
IC50 is 0.06 mM | Homo sapiens | 1,10-phenanthroline | |
3.4.11.7 | 0.065 | - |
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+ | Homo sapiens | angiotensin II |