Literature summary extracted from
Chang, F.J.; Lemme, S.; Sun, Q.; Sunahara, R.K.; Beuve, A.
Nitric oxide-dependent allosteric inhibitory role of a second nucleotide binding site in soluble guanylyl cyclase (2005), J. Biol. Chem., 280, 11513-11519.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.6.1.2 |
wild-type and mutant enzymes expressed in Sf21/baculovirus system |
Rattus norvegicus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.6.1.2 |
D477A |
mutation in beta subunit, mutant does not show a non-competitive mechanism with Mg2+GTPgammaS and Mg2+ATPgammaS that is observed with wild-type enzyme |
Rattus norvegicus |
4.6.1.2 |
E525K/C594D |
mutation in alpha subunit, mutant does not show a non-competitive mechanism with Mg2+GTPgammaS and Mg2+ATPgammaS that is observed with wild-type enzyme |
Rattus norvegicus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
adenosine-5'-tetraphosphate |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
4.6.1.2 |
ADPbetaS |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
4.6.1.2 |
GDPbetaS |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
4.6.1.2 |
guanosine-5'-tetraphosphate |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
4.6.1.2 |
ITP |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
4.6.1.2 |
Mg2+ATPgammaS |
inhibits cyclase activity through a mixed, non-competitive mechanism, only observable under NO stimulation and not under basal conditions |
Rattus norvegicus |
|
4.6.1.2 |
Mg2+GTPgammaS |
inhibits cyclase activity through a mixed, non-competitive mechanism, only observable under NO stimulation and not under basal conditions |
Rattus norvegicus |
|
4.6.1.2 |
XDP |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
4.6.1.2 |
XTP |
basal activity of wild-type enzyme is considerably less sensitive than NO-stimulated wild-type activity |
Rattus norvegicus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
0.021 |
- |
GTP |
pH 8.0, 30°C, wild-type enzyme, in presence of NO-donor S-nitroso-N-acetylpenicillamine |
Rattus norvegicus |
|
4.6.1.2 |
0.122 |
- |
GTP |
pH 8.0, 30°C, wild-type enzyme |
Rattus norvegicus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.2 |
Rattus norvegicus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.6.1.2 |
- |
Rattus norvegicus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.6.1.2 |
NO receptor |
alpha1*beta1 isoform of soluble guanylyl cyclase |
Rattus norvegicus |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
0.251 |
- |
Mg2+GTPgammaS |
pH 8.0, 30°C, wild-type enzyme |
Rattus norvegicus |
|