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Literature summary extracted from

  • Nelson, S.W.; Honzatko, R.B.; Fromm, H.J.
    Origin of cooperativity in the activation of fructose-1,6-bisphosphatase by Mg2+ (2004), J. Biol. Chem., 279, 18481-18487.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 D118A mutant tetramer with one wild-type subunit and three mutant subunits. Kinetic parameters similar to wild-type, kcat-value is about one-fourth that of wild-type Sus scrofa
3.1.3.11 D121A mutant tetramer with one wild-type subunit and three mutant subunits. Kinetic parameters similar to wild-type, kcat-value is about one-fourth that of wild-type Sus scrofa
3.1.3.11 E97A mutant tetramer with one wild-type subunit and three mutant subunits. Kinetic parameters similar to wild-type, kcat-value is about one-fourth that of wild-type Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP wild-type, 50% inhibition at 0.0016 mM, mutant E97A, 50% inhibition at 0.0038 mM, mutant D118A, 50% inhibition at 0.0069 mM, mutant D121A, 50% inhibition at 0.0074 mM Sus scrofa
3.1.3.11 D-fructose 2,6-bisphosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 additional information
-
 additional information enzyme shows a mechanism of cooperativity that arises from within a single subunit Sus scrofa
3.1.3.11 0.0011
-
 D-fructose 1,6-bisphosphate mutant D121A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 0.0013
-
 D-fructose 1,6-bisphosphate mutant D118A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 0.0018
-
 D-fructose 1,6-bisphosphate wild-type, pH 7.5 Sus scrofa
3.1.3.11 0.0018
-
 D-fructose 1,6-bisphosphate mutant E97A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 0.0022
-
 D-fructose 1,6-bisphosphate wild-type, E-tagged, pH 7.5 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Sus scrofa P00636
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Sus scrofa D-fructose 6-phosphate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 3.9
-
 D-fructose 1,6-bisphosphate mutant D118A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 5.3
-
 D-fructose 1,6-bisphosphate mutant E97A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 5.8
-
 D-fructose 1,6-bisphosphate mutant D121A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 22
-
 D-fructose 1,6-bisphosphate wild-type, pH 7.5 Sus scrofa
3.1.3.11 23
-
 D-fructose 1,6-bisphosphate wild-type, E-tagged, pH 7.5 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.11 0.00011
-
 D-fructose 2,6-bisphosphate wild-type, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 0.00012
-
 D-fructose 2,6-bisphosphate wild-type, pH 7.5 Sus scrofa
3.1.3.11 0.00016
-
 D-fructose 2,6-bisphosphate mutant E97A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 0.00017
-
 D-fructose 2,6-bisphosphate mutant D118A, E-tagged, pH 7.5 Sus scrofa
3.1.3.11 0.00061
-
 D-fructose 2,6-bisphosphate mutant D121A, E-tagged, pH 7.5 Sus scrofa