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Literature summary extracted from

  • Choi, K.H.; Kremer, L.; Besra, G.S.; Rock, C.O.
    Identification and substrate specificity of beta-ketoacyl (acyl carrier protein) synthase III (mtFabH) from Mycobacterium tuberculosis (2000), J. Biol. Chem., 275, 28201-28207.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 gene fabH, overexpression of His-tagged enzyme in Mycobacterium bovis strain BCG Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.180 ampicillin inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
2.3.1.180 Ethionamide inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
2.3.1.180 isoniazid inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
2.3.1.180 isoxyl inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
2.3.1.180 additional information the mitochondrial enzyme is not affected by cerulenin Mycobacterium tuberculosis
2.3.1.180 rifampicin inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
2.3.1.180 thiolactomycin inhibition of the purified enzyme in vitro, and of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.180 43000
-
 x * 43000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis the condensation reaction is the first step in elongation in fatty acid synthesis by fatty acid synthase complex acetoacyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 recombinant His-tagged enzyme from Mycobacterium bovis strain BCG Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 active site structure a catayltic triad of Cys-His-Asn residues Mycobacterium tuberculosis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 additional information
-
 substrate specificity of the purified His-tagged enzyme Mycobacterium tuberculosis
2.3.1.180 0.00013
-
 purified His-tagged enzyme, substrate lauroyl-CoA Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acyl-CoA + malonyl-[acyl-carrier protein] the condensation reaction is the first step in elongation in fatty acid synthesis by fatty acid synthase complex Mycobacterium tuberculosis acetoacyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 arachidoyl-CoA + malonyl-[acyl-carrier protein] low activity Mycobacterium tuberculosis 3-oxo-docosanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 decanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis 3-oxo-lauroyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 lauroyl-CoA + malonyl-[acyl-carrier protein] lauroyl-CoA is the preferred primer Mycobacterium tuberculosis 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information substrate specificity, no activity with isovaleryl-CoA, butyryl-CoA, and acetyl-CoA, the enzyme prefers long chain acyl-CoAs as primers rather than acyl-ACPs Mycobacterium tuberculosis ?
-
 ?
2.3.1.180 myristoyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis 3-oxo-palmitoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 octanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 palmitoyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis 3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 stearoyl-CoA + malonyl-[acyl-carrier protein] low activity Mycobacterium tuberculosis 3-oxo-arachidoyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.180 ? x * 43000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl (acyl carrier protein) synthase III
-
 Mycobacterium tuberculosis
2.3.1.180 beta-ketoacyl-ACP III
-
 Mycobacterium tuberculosis
2.3.1.180 FabH
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7
-
 assay at Mycobacterium tuberculosis