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Literature summary extracted from

  • Yamamoto, S.; Hayaishi, O.
    Tryptophan pyrrolase of rabbit intestine (1967), J. Biol. Chem., 242 (22), 5260-5266.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 CO reversed by illumination Oryctolagus cuniculus
1.13.11.52 hydroxylamine sulfate 0.1 mM causes 79% inhibition, 1 mM causes 94% inhibition Oryctolagus cuniculus
1.13.11.52 KCN
-
Oryctolagus cuniculus
1.13.11.52 L-tryptophan substrate inhibition Oryctolagus cuniculus
1.13.11.52 additional information not inhibited by catalase and D-tryptophan Oryctolagus cuniculus
1.13.11.52 Sodium azide 1 mM causes 20% inhibition Oryctolagus cuniculus
1.13.11.52 Tiron 1 mM causes 40% inhibition Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.52 D-tryptophan + O2 Oryctolagus cuniculus
-
N-formyl-D-kynurenine
-
?
1.13.11.52 L-tryptophan + O2 Oryctolagus cuniculus
-
N-formyl-L-kynurenine
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52 homogenization, ammonium sulfate precipitation Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.52 intestine
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.52 0.01
-
ascending colon, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 0.01
-
caecum, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 0.04
-
small intestine IV, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 0.05
-
small intestine proximal portion, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 0.07
-
small intestine V, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 0.08
-
appendix, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 0.15
-
appendix, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 5.19
-
small intestine VI, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 6.97
-
small intestine distal portion, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus
1.13.11.52 234
-
enzyme after purification, 0.05 mM potassium phosphate buffer (pH 7.5), 0.005 mM methylene blue, 0.01 mM ascorbic acid, 0.003 mM tryptophan, 37°C Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 D-tryptophan + O2
-
Oryctolagus cuniculus N-formyl-D-kynurenine
-
?
1.13.11.52 L-tryptophan + O2
-
Oryctolagus cuniculus N-formyl-L-kynurenine
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.52 6.5
-
L-tryptophan Oryctolagus cuniculus
1.13.11.52 7.5
-
D-tryptophan Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.52 5.5 8 5% activity loss at pH 5.5, 10% activity loss at pH 7, 20% activity loss at pH 8 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 methylene blue stimulates the formation of D-kynurenine Oryctolagus cuniculus
1.13.11.52 additional information ascorbic acid, can be replaced by xanthine oxidase with hypoxanthine Oryctolagus cuniculus
1.13.11.52 additional information not affected by hydrogen peroxide Oryctolagus cuniculus
1.13.11.52 additional information toluidine blue, can replace methylene blue Oryctolagus cuniculus