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Literature summary extracted from

  • El-Bessoumy, A.A.; Sarhan, M.; Mansour, J.
    Production, isolation, and purification of L-asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation (2004), J. Biochem. Mol. Biol., 37, 387-393.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 medicine used for treatment of acute lymphoblastic leukemia, pancreatic carcinoma, and bovine lymphosarcoma Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.147
-
 L-Asn
-
 Pseudomonas aeruginosa
3.5.1.1 0.147
-
 L-asparagine pH 9.0, 37°C Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 160000
-
 gel filtration Pseudomonas aeruginosa
3.5.1.1 160000
-
 SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-Asn + H2O Pseudomonas aeruginosa
-
 L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O Pseudomonas aeruginosa 50071
-
 L-Asp + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Pseudomonas aeruginosa
-
 L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Pseudomonas aeruginosa 50071
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pseudomonas aeruginosa
-
-
-
3.5.1.1 Pseudomonas aeruginosa
-
 solid state fermentation
-
3.5.1.1 Pseudomonas aeruginosa 50071
-
-
-
3.5.1.1 Pseudomonas aeruginosa 50071
-
 solid state fermentation
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Pseudomonas aeruginosa
3.5.1.1 native enzyme from strain 50071, grown on solid-state fermentation, 106fold to homogeneity by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 1900
-
 purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-Asn + H2O
-
 Pseudomonas aeruginosa L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pseudomonas aeruginosa 50071 L-Asp + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Pseudomonas aeruginosa L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Pseudomonas aeruginosa 50071 L-aspartate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 monomer 1 * 160000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 35 40
-
 Pseudomonas aeruginosa
3.5.1.1 37
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 25 60
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 9
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 4 11
-
 Pseudomonas aeruginosa