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Literature summary extracted from

  • Roberts, J.; Burson, G.; Hill, J.M.
    New procedures for purification of L-asparaginase with high yield from Escherichia coli (1968), J. Bacteriol., 95, 2117-2123.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.1 medicine potent antineoplastic agent in animals which has given complete remission in some human leukemias Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-Asn + H2O Escherichia coli
-
 L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O Escherichia coli HAP
-
 L-Asp + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Escherichia coli
-
 highest yield in aerobically grown cells in corn steep medium, addition of L-glutamic acid, L-methionine, and lactic acid enhances enzyme production, addition of glucose or sodium depresses enzyme production
-
3.5.1.1 Escherichia coli HAP
-
 highest yield in aerobically grown cells in corn steep medium, addition of L-glutamic acid, L-methionine, and lactic acid enhances enzyme production, addition of glucose or sodium depresses enzyme production
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 620
-
 pH 8.0, 37°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 5°C lyphilyzed enzyme, stable upon storage for at least several weeks Escherichia coli
3.5.1.1 5°C, sterile enzyme solution with high specific activity, several weeks, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-Asn + H2O
-
 Escherichia coli L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Escherichia coli HAP L-Asp + NH3
-
 ?