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Literature summary extracted from

  • Jobin, M.C.; Martinez, G.; Motard, J.; Gottschalk, M.; Grenier, D.
    Cloning, purification, and enzymatic properties of dipeptidyl peptidase IV from the swine pathogen Streptococcus suis (2005), J. Bacteriol., 187, 795-799.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.14.5 aminoethyl-benzene sulfonyl fluoride hydrochloride 1 mM, 18% activation Streptococcus suis
3.4.14.5 N-(trans-epoxysuccinyl)-L-leucine-4-guanidinobutylamide 0.01 mM, 25% activation Streptococcus suis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 overexpression in Escherichia coli Streptococcus suis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 1,10-phenanthroline 10 mM, 34% inhibition Streptococcus suis
3.4.14.5 3,4-dichloroisocoumarin 0.1 mM, 18% inhibition Streptococcus suis
3.4.14.5 Diprotin A 1 mM, 71% inhibition Streptococcus suis
3.4.14.5 Hg2+ 1 mM, 70% inhibition Streptococcus suis
3.4.14.5 iodoacetamide 0.01 mM, 19% inhibition Streptococcus suis
3.4.14.5 SDS 35 mM, complete inhibition Streptococcus suis
3.4.14.5 Triton X-100 16 mM, 22% inhibition Streptococcus suis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 0.26
-
Gly-Pro-4-nitroanilide
-
Streptococcus suis

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Streptococcus suis Q673U2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
Streptococcus suis

Storage Stability

EC Number Storage Stability Organism
3.4.14.5 -20°C, stable to prolonged storage Streptococcus suis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Ala-4-nitroanilide + H2O 36% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Ala-Ala + 4-nitroaniline
-
?
3.4.14.5 Ala-Pro-4-nitroanilide + H2O 80% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Ala-Pro + 4-nitroaniline
-
?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O 42% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Arg-Pro + 4-nitroaniline
-
?
3.4.14.5 Asp-Pro-4-nitroanilide + H2O 11% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Asp-Pro + 4-nitroaniline
-
?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
Streptococcus suis Gly-Pro + 4-nitroaniline
-
?
3.4.14.5 Lys-Pro-4-nitroanilide + H2O 51% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Lys-Pro + 4-nitroaniline
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 37
-
-
Streptococcus suis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.14.5 24 45 24°C: 52% of maximal activity, 45°C: 78% of maximal activity Streptococcus suis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 4
-
24 h, 86% loss of activity, diluted preparation of recombinant enzyme Streptococcus suis
3.4.14.5 10
-
24 h, 92% loss of activity, diluted preparation of recombinant enzyme Streptococcus suis
3.4.14.5 24
-
24 h, 93% loss of activity, diluted preparation of recombinant enzyme Streptococcus suis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 8
-
-
Streptococcus suis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 6 9 pH 6: 61% of maximal activity, pH 9: 54% of maximal activity Streptococcus suis