EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.21 | expression in Escherichia coli | Leptospira interrogans |
2.3.3.21 | gene cimA, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli | Leptospira interrogans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.21 | isoleucine | - |
Leptospira interrogans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 0.04 | - |
pyruvate | recombinant enzyme, pH 8.0, 37°C | Leptospira interrogans | |
2.3.3.21 | 0.043 | - |
pyruvate | - |
Leptospira interrogans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 60000 | - |
x * 60000, recombinant His6-tagged enzyme, SDS-PAGE | Leptospira interrogans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.21 | acetyl-CoA + pyruvate + H2O | Leptospira interrogans | the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine | CoA + (R)-citramalate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.21 | Leptospira interrogans | - |
serotype lai strain 56601 | - |
2.3.3.21 | Leptospira interrogans | - |
serotype lai strain 56601, gene cimA or LA2350 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.21 | - |
Leptospira interrogans |
2.3.3.21 | recombinant His6-tagged enzyme 14.3fold from Escherichia coli by nickel affinity chromatography to homogeneity | Leptospira interrogans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 0.174 | - |
crude extract of Escherichia coli recombinantly expressing CimA | Leptospira interrogans |
2.3.3.21 | 2.5 | - |
- |
Leptospira interrogans |
2.3.3.21 | 2.53 | - |
purified recombinant His6-tagged enzyme | Leptospira interrogans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.21 | acetyl-CoA + pyruvate | the enzyme is strictly specific for pyruvate as keto acid substrate | Leptospira interrogans | (3S)-citramalyl-CoA | - |
? | |
2.3.3.21 | acetyl-CoA + pyruvate + H2O | the enzyme is involved in the threonine-independent biosynthesis of isoleucine via an alternative beta-methyl-D-malate pathway, the expression of cimA is transcriptionally regulated by isoleucine | Leptospira interrogans | CoA + (R)-citramalate | - |
? | |
2.3.3.21 | acetyl-CoA + pyruvate + H2O | the enzyme is strictly specific for pyruvate as the keto acid substrate | Leptospira interrogans | CoA + (R)-citramalate | - |
? | |
2.3.3.21 | additional information | LA2350 is a citramalate synthase without detectable alpha-isopropylmalate synthase activity | Leptospira interrogans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.21 | ? | x * 60000, recombinant His6-tagged enzyme, SDS-PAGE | Leptospira interrogans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.21 | CimA | - |
Leptospira interrogans |
2.3.3.21 | citramalate synthase | - |
Leptospira interrogans |
2.3.3.21 | LA2350 | - |
Leptospira interrogans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 37 | - |
- |
Leptospira interrogans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | additional information | - |
temperature profile, overview | Leptospira interrogans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | - |
40 | 80% of maximal activity within this range | Leptospira interrogans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.21 | 2.41 | - |
pyruvate | - |
Leptospira interrogans | |
2.3.3.21 | 2.41 | - |
pyruvate | recombinant enzyme, pH 8.0, 37°C | Leptospira interrogans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 8 | - |
assay at | Leptospira interrogans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.21 | acetyl-CoA | - |
Leptospira interrogans |