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Literature summary extracted from

  • Mori, H.; Shibasaki, T.; Yano, K.; Ozaki, A.
    Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline (1997), J. Bacteriol., 179, 5677-5683.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.28 L-ascorbate 2 mM, no further stimulation above 2 mM Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.28 Citric acid 30% inhibition at 5 mM Streptomyces sp.
1.14.11.28 Co2+ more than 90% inhibition at 1 mM Streptomyces sp.
1.14.11.28 Cu2+ more than 90% inhibition at 1 mM Streptomyces sp.
1.14.11.28 EDTA 95% inhibition at 2 mM Streptomyces sp.
1.14.11.28 Zn2+ more than 90% inhibition at 1 mM Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.28 0.11
-
 2-oxoglutarate 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C Streptomyces sp.
1.14.11.28 0.56
-
 L-proline 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.28 Fe2+ strictly required, optimal stimulation at 1 mM Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.28 35000
-
 SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.28 Streptomyces sp.
-
 strainTH1
-
1.14.11.28 Streptomyces sp. TH1
-
 strainTH1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.28 5 chromatographic steps near to homogeneity Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.28 cell culture
-
 Streptomyces sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.28 proline 3-hydroxylase
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.28 35
-
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.28 3.2
-
 L-proline 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.28 7
-
-
 Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.28 2-oxoglutarate strictly required, highest activity at 4 mM Streptomyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.11.28 Streptomyces sp.
-
-
 4.3