Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Broadbent, J.R.; Rodriguez, B.T.; Joseph, P.; Smith, E.A.; Steele, J.L.
    Conversion of Lactococcus lactis cell envelope proteinase specificity by partial allele exchange (2006), J. Appl. Microbiol., 100, 1307-1317.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.96 biotechnology conversion of lactocepin substrate binding regions by allele exchange can effectively alter lactocepin specificity in industrial strains of Lactococcus lactis without significantly affecting other important strain properties. The methodology can be used to alter lactocepin specificity in commercial starter cultures with a propensity for bitter flavour defect Lactococcus lactis
3.4.21.96 industry method to alter lactocepin specificity in commercial starter cultures with a propensity for bitter flavour defect. PrtP derivatives developed by this approach should be suitable for commercial applications in the US and other countries with a favourable regulatory climate Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.96 additional information prtP replacement mutants (strains S3 or S4 with altered lactocepin gene sequence) have a slower rate of acidification than parental strains after 2.5 or 5 h incubation in ultra high temperature skim milk Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.96 Lactococcus lactis
-
 commercial cheese starter culture, group h lactocepin
-
3.4.21.96 Lactococcus lactis Q49SG9
-
-
3.4.21.96 Lactococcus lactis Q49SH0
-
-
3.4.21.96 Lactococcus lactis s4
-
 commercial cheese starter culture, group h lactocepin
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.96 alpha(S1)-casein + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.21.96 alphas1-Casein fragment 1-23 + H2O
-
 Lactococcus lactis Fragments of alphas1-casein fragment 1-23
-
 ?
3.4.21.96 alphas1-Casein fragment 1-23 + H2O
-
 Lactococcus lactis s4 Fragments of alphas1-casein fragment 1-23
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.96 cell envelope proteinase
-
 Lactococcus lactis