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Literature summary extracted from

  • Turner, A.J.
    Membrane alanyl aminopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 289-294.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.11.2 diagnostics the enzyme might be a valuable histological marker for prostate cancer Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 DNA and amino acid sequence determination and analysis Rattus norvegicus
3.4.11.2 from a kidney library Oryctolagus cuniculus
3.4.11.2 from an intestinal cDNA library, the gene is localized on chromosome 15, intestinal and myeloid epithelial cells use different promoters Homo sapiens
3.4.11.2 the gene is localized on chromosome 7 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 actinonin IC50: 0.002 mM, specific inhibitor Sus scrofa
3.4.11.2 amastatin slow, tight-binding inhibitor which involves a conformational change in the enzyme-inhibitor complex Sus scrofa
3.4.11.2 bestatin
-
 Sus scrofa
3.4.11.2 Metal chelating agents
-
 Sus scrofa
3.4.11.2 additional information no inhibition by sulfhydryl reagents Sus scrofa
3.4.11.2 probestin IC50: 50 nM Sus scrofa
3.4.11.2 puromycin very weak inhibition Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 cell surface CD13 Homo sapiens 9986
-
3.4.11.2 membrane
-
 Lactococcus lactis 16020
-
3.4.11.2 membrane
-
 Lymantria dispar 16020
-
3.4.11.2 membrane brush border membranes Homo sapiens 16020
-
3.4.11.2 membrane brush border membranes Rattus norvegicus 16020
-
3.4.11.2 membrane brush border membranes Sus scrofa 16020
-
3.4.11.2 membrane brush border membranes Oryctolagus cuniculus 16020
-
3.4.11.2 microsome
-
 Homo sapiens
-
-
3.4.11.2 microsome
-
 Rattus norvegicus
-
-
3.4.11.2 microsome
-
 Sus scrofa
-
-
3.4.11.2 microsome
-
 Oryctolagus cuniculus
-
-
3.4.11.2 plasma membrane type II integral membrane protein located on the plasma membrane as an ectoenzyme Homo sapiens 5886
-
3.4.11.2 plasma membrane type II integral membrane protein located on the plasma membrane as an ectoenzyme Rattus norvegicus 5886
-
3.4.11.2 plasma membrane type II integral membrane protein located on the plasma membrane as an ectoenzyme Sus scrofa 5886
-
3.4.11.2 plasma membrane type II integral membrane protein located on the plasma membrane as an ectoenzyme Oryctolagus cuniculus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Zn2+ the enzyme contains a typical zinc-binding motif HEXXH, one Zn2+ per subunit Rattus norvegicus
3.4.11.2 Zn2+ zinc-metallopeptidase, Zn2+ binds to the active site Homo sapiens
3.4.11.2 Zn2+ zinc-metallopeptidase, Zn2+ binds to the active site Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 130000
-
 kidney brush border enzyme, gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 additional information Sus scrofa the brain enzyme is involved in termination of the action of neuropeptides, especially of enkephalins and angiotensin III, the kidney enzyme contributes to the extracellular catabolism of glutathione, the intestinal enzyme acts as a receptor for the transmissible gastroenteritis virus, a coronavirus, causing fatal diarrhea in newborn pigs ?
-
 ?
3.4.11.2 additional information Lymantria dispar the enzyme acts as major receptor for the CryIAc toxin of Bacillus thuringiensis ?
-
 ?
3.4.11.2 additional information Homo sapiens the enzyme in granulocytes may cooperate with neprilysin, the enzyme is involved in the metabolism of certain vasoactive peptides in vascular cells in the brain, the enzyme mediates human cytomegalovirus infection, the enzym regulates the cycle-dependent bioavailability of interleukin-8 in the endometrium, the enzyme's activity is regulated by estrogen ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Homo sapiens
-
-
-
3.4.11.2 Lactococcus lactis
-
 gene pepN
-
3.4.11.2 Lymantria dispar
-
 gypsy moth
-
3.4.11.2 Oryctolagus cuniculus
-
-
-
3.4.11.2 Rattus norvegicus
-
-
-
3.4.11.2 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.2 glycoprotein
-
 Homo sapiens
3.4.11.2 glycoprotein
-
 Oryctolagus cuniculus
3.4.11.2 glycoprotein heavily glycosylated Sus scrofa
3.4.11.2 glycoprotein the enzyme contains 9 potential N-glycosylation sites Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 native enzyme from kidney and from small intestine, the kidney brush border enzyme is purified by proteinase treatment or detergent solubilization and chromatographical steps, e.g. affinity chromatography on a 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate resin followed by a concanavalin A resin, the small intestinal enzyme is purified by immunoadsorbent chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 astrocyte
-
 Sus scrofa
-
3.4.11.2 blood vessel lung endothelium Sus scrofa
-
3.4.11.2 blood vessel endothelium lung blood vessels Sus scrofa
-
3.4.11.2 brain
-
 Homo sapiens
-
3.4.11.2 brain endothelial cells, synaptic membranes of astro- and pericytes Sus scrofa
-
3.4.11.2 granulocyte
-
 Homo sapiens
-
3.4.11.2 hematopoietic cell CD13 Homo sapiens
-
3.4.11.2 hematopoietic stem cell
-
 Homo sapiens
-
3.4.11.2 intestine
-
 Homo sapiens
-
3.4.11.2 kidney
-
 Sus scrofa
-
3.4.11.2 kidney
-
 Oryctolagus cuniculus
-
3.4.11.2 liver
-
 Sus scrofa
-
3.4.11.2 lung located on endothelial cells in blood vessels Sus scrofa
-
3.4.11.2 lymphocyte
-
 Homo sapiens
-
3.4.11.2 melanoma cell
-
 Homo sapiens
-
3.4.11.2 monocyte
-
 Homo sapiens
-
3.4.11.2 additional information wide tissue distribution Homo sapiens
-
3.4.11.2 additional information wide tissue distribution Rattus norvegicus
-
3.4.11.2 additional information wide tissue distribution Sus scrofa
-
3.4.11.2 additional information wide tissue distribution Oryctolagus cuniculus
-
3.4.11.2 mucosa
-
 Sus scrofa
-
3.4.11.2 myeloid leukemia cell
-
 Homo sapiens
-
3.4.11.2 pericyte
-
 Sus scrofa
-
3.4.11.2 placenta
-
 Sus scrofa
-
3.4.11.2 prostate cancer cell
-
 Homo sapiens
-
3.4.11.2 small intestine mucosa Sus scrofa
-
3.4.11.2 T-lymphocyte activated Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Cys-Gly + H2O
-
 Homo sapiens Cys + Gly
-
 ?
3.4.11.2 Cys-Gly + H2O
-
 Rattus norvegicus Cys + Gly
-
 ?
3.4.11.2 Cys-Gly + H2O
-
 Sus scrofa Cys + Gly
-
 ?
3.4.11.2 Cys-Gly + H2O
-
 Oryctolagus cuniculus Cys + Gly
-
 ?
3.4.11.2 enkephalin + H2O hydrolysis of the Tyr1-Gly2 bond Homo sapiens ?
-
 ?
3.4.11.2 enkephalin + H2O hydrolysis of the Tyr1-Gly2 bond Rattus norvegicus ?
-
 ?
3.4.11.2 enkephalin + H2O hydrolysis of the Tyr1-Gly2 bond Sus scrofa ?
-
 ?
3.4.11.2 enkephalin + H2O hydrolysis of the Tyr1-Gly2 bond Oryctolagus cuniculus ?
-
 ?
3.4.11.2 L-Ala-2-naphthylamide + H2O
-
 Homo sapiens L-Ala + 2-naphthylamine
-
 ?
3.4.11.2 L-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus L-Ala + 2-naphthylamine
-
 ?
3.4.11.2 L-Ala-2-naphthylamide + H2O
-
 Sus scrofa L-Ala + 2-naphthylamine
-
 ?
3.4.11.2 L-Ala-2-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Ala + 2-naphthylamine
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Homo sapiens L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Sus scrofa L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Oryctolagus cuniculus L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Sus scrofa L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Oryctolagus cuniculus L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 additional information the brain enzyme is involved in termination of the action of neuropeptides, especially of enkephalins and angiotensin III, the kidney enzyme contributes to the extracellular catabolism of glutathione, the intestinal enzyme acts as a receptor for the transmissible gastroenteritis virus, a coronavirus, causing fatal diarrhea in newborn pigs Sus scrofa ?
-
 ?
3.4.11.2 additional information the enzyme acts as major receptor for the CryIAc toxin of Bacillus thuringiensis Lymantria dispar ?
-
 ?
3.4.11.2 additional information the enzyme in granulocytes may cooperate with neprilysin, the enzyme is involved in the metabolism of certain vasoactive peptides in vascular cells in the brain, the enzyme mediates human cytomegalovirus infection, the enzym regulates the cycle-dependent bioavailability of interleukin-8 in the endometrium, the enzyme's activity is regulated by estrogen Homo sapiens ?
-
 ?
3.4.11.2 additional information substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide Homo sapiens ?
-
 ?
3.4.11.2 additional information substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide Rattus norvegicus ?
-
 ?
3.4.11.2 additional information substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide Oryctolagus cuniculus ?
-
 ?
3.4.11.2 additional information substrate specificity, overview, chain length influencs the activity rate, dipeptides are readily hydrolyzed, the enzyme prefers substrates with N-terminal L-Ala residues, the specificity for N-terminal residues is descending order is Ala, Phe, Tyr, Leu, Arg, Thr, Trp, Lys, Ser, Asp, His, Val. Pro- and alpha- or gamma-Glu-derivatives are slowly hydrolyzed, when a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide. Glu350 is involved in catalysis, the enzyme in lung is identical with the p146 type II alveolar epithelial cell antigen Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer
-
 Oryctolagus cuniculus
3.4.11.2 More the enzyme contains a small cytoplasmic domain, a 24-amino-acid hydrophobic segment close to the N-terminus which serves as membrane anchor, and the bulk domain including the active site as ectodomain Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase M
-
 Sus scrofa
3.4.11.2 aminopeptidase N
-
 Homo sapiens
3.4.11.2 aminopeptidase N
-
 Rattus norvegicus
3.4.11.2 aminopeptidase N
-
 Sus scrofa
3.4.11.2 aminopeptidase N
-
 Oryctolagus cuniculus
3.4.11.2 aminopeptidase N
-
 Lactococcus lactis
3.4.11.2 aminopeptidase N
-
 Lymantria dispar
3.4.11.2 CD13 cluster differentiation antigen Homo sapiens
3.4.11.2 Cys-Gly-dipeptidase
-
 Sus scrofa
3.4.11.2 cysteinyl-glycinase
-
 Sus scrofa
3.4.11.2 mAAP
-
 Homo sapiens
3.4.11.2 mAAP
-
 Rattus norvegicus
3.4.11.2 mAAP
-
 Sus scrofa
3.4.11.2 mAAP
-
 Oryctolagus cuniculus
3.4.11.2 mAAP
-
 Lactococcus lactis
3.4.11.2 mAAP
-
 Lymantria dispar
3.4.11.2 p146 type II alveolar epithelial cell antigen
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7 7.5 dependent on substrate concentration Homo sapiens
3.4.11.2 7 7.5 dependent on substrate concentration Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.2 0.00002
-
 amastatin preincubation of the enzyme with the inhibitor Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.2 Sus scrofa about
-
 5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.2 0.00005
-
 IC50: 50 nM Sus scrofa probestin
3.4.11.2 0.002
-
 IC50: 0.002 mM, specific inhibitor Sus scrofa actinonin