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Literature summary extracted from

  • Adachi, O.
    Tryptophanyl aminopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2nd. ed., 1014-1015.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.11.17 synthesis the enzyme is useful for synthesis of L-Trp because it is not inhibited by high levels of the product Cutaneotrichosporon cutaneum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.17
-
 Cutaneotrichosporon cutaneum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.17 2,2'-dipyridyl
-
 Cutaneotrichosporon cutaneum
3.4.11.17 N-ethylmaleimide
-
 Cutaneotrichosporon cutaneum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.17 cytosol
-
 Cutaneotrichosporon cutaneum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.17 Co2+ 25% of the activation caused by Mn2+ Cutaneotrichosporon cutaneum
3.4.11.17 Mn2+ above 2.5 mM, required Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.17 68000
-
 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
3.4.11.17 270000
-
 gel filtration Cutaneotrichosporon cutaneum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.17 Cutaneotrichosporon cutaneum
-
 IFO 0173
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.17
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.17 Ala-amide + H2O 14% of the activity with Trp-amide Cutaneotrichosporon cutaneum Ala + NH3
-
 ?
3.4.11.17 Asn + H2O 18% of the activity with Trp-amide Cutaneotrichosporon cutaneum Asp + NH3
-
 ?
3.4.11.17 Gln + H2O 18% of the activity with Trp-amide Cutaneotrichosporon cutaneum Glu + NH3
-
 ?
3.4.11.17 L-citrulline + H2O 22% of the activity with Trp-amide Cutaneotrichosporon cutaneum (2S)-2-amino-5-(carboxyamino)pentanoic acid + NH3
-
 ?
3.4.11.17 Leu-amide + H2O 26% of the activity with Trp-amide Cutaneotrichosporon cutaneum Leu + NH3
-
 ?
3.4.11.17 Met-amide + H2O 14% of the activity with Trp-amide Cutaneotrichosporon cutaneum Met + NH3
-
 ?
3.4.11.17 Phe-amide + H2O 47% of the activity with Trp-amide Cutaneotrichosporon cutaneum Phe + NH3
-
 ?
3.4.11.17 Ser-amide + H2O 7% of the activity with Trp-amide Cutaneotrichosporon cutaneum Ser + NH3
-
 ?
3.4.11.17 Trp-amide + H2O
-
 Cutaneotrichosporon cutaneum Trp + NH3
-
 ?
3.4.11.17 Tyr-amide + H2O 22% of the activity with Trp-amide Cutaneotrichosporon cutaneum Tyr + NH3
-
 ?
3.4.11.17 Val-amide + H2O 15% of the activity with Trp-amide Cutaneotrichosporon cutaneum Val + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.17 tetramer 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.17 40 45 at pH 7.5 Cutaneotrichosporon cutaneum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.17 55
-
 10 min, stable up to Cutaneotrichosporon cutaneum
3.4.11.17 60
-
 heating above causes rapid inactivation Cutaneotrichosporon cutaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.17 9 9.5
-
 Cutaneotrichosporon cutaneum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.17 7.5 8.5 20 h, stable at room temperature Cutaneotrichosporon cutaneum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.17 Cutaneotrichosporon cutaneum isoelectric focusing
-
 4.7