Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Woessner, J.F.
    Clostridial aminopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2nd. Ed., 1011-1012.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.11.13 not stable to lyophilization Hathewaya histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.13 Cu2+ inhibits in presence of Mg2+ Hathewaya histolytica
3.4.11.13 Lys(Abz)-Phe-p-nitrobenzyloxy
-
 Hathewaya histolytica
3.4.11.13 p-mercuribenzoate 1.7 mM, complete inhibition Hathewaya histolytica
3.4.11.13 Zn2+ inhibits in presence of Mg2+ Hathewaya histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.13 Co2+ activates to a lesser extent than Mn2+, no activity in absence of metal ions Hathewaya histolytica
3.4.11.13 Mn2+ activates, no activity in absence of metal ions Hathewaya histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.13 56000
-
 6 * 56000, SDS-PAGE Hathewaya histolytica
3.4.11.13 340000
-
 gel filtration Hathewaya histolytica

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.13 Hathewaya histolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.13
-
 Hathewaya histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.13 culture medium
-
 Hathewaya histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.13 (Pro-Gly-Pro)n + H2O
-
 Hathewaya histolytica Pro + Gly-Pro-(Pro-Gly-Pro)n-1
-
 ?
3.4.11.13 (Pro-Gly-Pro)n-methyl ester + H2O
-
 Hathewaya histolytica Pro + Gly-Pro-(Pro-Gly-Pro)n-methyl ester
-
 ?
3.4.11.13 additional information enzyme can not remove the terminal Pro from poly-L-Pro. The enzyme is strictly an aminopeptidase and has no endopeptidase action on polypeptides and proteins. The amino group of the N-terminal amino acid must be free. Di-, tri- and polypeptide substrates are cleaved and the optimum size accomodated by the active center is four residues. The residues cleaved most rapidly are hydrophobic ones Hathewaya histolytica ?
-
 ?
3.4.11.13 Pro-Gly-Pro + H2O
-
 Hathewaya histolytica Pro + Gly-Pro
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.13 hexamer 6 * 56000, SDS-PAGE Hathewaya histolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.13 0.003
-
 Lys(Abz)-Phe-p-nitrobenzyloxy
-
 Hathewaya histolytica