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Literature summary extracted from

  • Ruemenapf, T.; Thiel, H.J.
    434. Cathepsin T (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 2nd Ed., 1404.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.3 cysteine the enzyme requires activation by reducing agents, optimal at 4 mM Ficus glabrata
3.4.22.24 DTT activates Rattus norvegicus
3.4.22.24 EDTA activates Rattus norvegicus
3.4.22.24 additional information carbon tetrachloride induces the enzyme 7fold in the liver Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.3 cystatin from egg white, strong inhibition Ficus glabrata
3.4.22.24 leupeptin
-
 Rattus norvegicus
3.4.22.24 tosyl-Lys-methylchloride
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.3 0.0024
-
 benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin pH 6.8, 37°C Ficus glabrata
3.4.22.3 0.3
-
 benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) pH 6.8, 37°C Ficus glabrata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.3 extracellular
-
 Ficus glabrata
-
-
3.4.22.24 lysosome
-
 Rattus norvegicus 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.3 additional information Ficus glabrata ficain is the major proteolytic activity component in the milky latex of the plant ?
-
 ?
3.4.22.24 tyrosine aminotransferase + H2O Rattus norvegicus 53 kDa substrate monomer, cleavage at the N-terminus of the protein, interconversion of the 3 different forms of the substrate enzyme without alteration of its enzymatic activity related to the substrate's short half-life in the cell truncated tyrosine aminotransferase + peptide 49 kDa product monomer and 4.5 kDa N-terminal peptide ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.3 Ficus glabrata
-
-
-
3.4.22.24 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.3 further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide Ficus glabrata
3.4.22.24 native enzyme to homogeneity from liver Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.3 Similar to that of papain active site structure Ficus glabrata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.3 commercial preparation needs further purification Ficus glabrata
-
3.4.22.3 fruit unripe, the enzyme is located in the latex milk secreted by the organ Ficus glabrata
-
3.4.22.3 latex
-
 Ficus glabrata
-
3.4.22.3 leaf the enzyme is located in the latex milk secreted by the organ Ficus glabrata
-
3.4.22.3 stem the enzyme is located in the latex milk secreted by the organ Ficus glabrata
-
3.4.22.24 kidney high activity Rattus norvegicus
-
3.4.22.24 liver
-
 Rattus norvegicus
-
3.4.22.24 lung
-
 Rattus norvegicus
-
3.4.22.24 small intestine
-
 Rattus norvegicus
-
3.4.22.24 spleen
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.3 benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O
-
 Ficus glabrata benzoyl-Phe-Val-Arg + 4-nitrophenol
-
 ?
3.4.22.3 benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O
-
 Ficus glabrata benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.3 benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O
-
 Ficus glabrata benzyloxycarbonyl-Phe-Arg + 4-nitrophenol
-
 ?
3.4.22.3 benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O
-
 Ficus glabrata benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.3 insulin B chain + H2O hydrolysis of 6 bonds Ficus glabrata fragments of insulin B chain
-
 ?
3.4.22.3 additional information ficain is the major proteolytic activity component in the milky latex of the plant Ficus glabrata ?
-
 ?
3.4.22.3 additional information substrate specificity compared to papain, the ficain S1-pocket accepts Gly, SEr, Glu, Tyr, and Phe, the S2-pocket accepts cysteic acid, Val, Leu, Gly, and Phe, Arg-NH-nitrophenyl is a poor substrate Ficus glabrata ?
-
 ?
3.4.22.24 azocasein + H2O degradation Rattus norvegicus ?
-
 ?
3.4.22.24 Hemoglobin + H2O denaturation Rattus norvegicus ?
-
 ?
3.4.22.24 tyrosine aminotransferase + H2O 53 kDa substrate monomer, cleavage at the N-terminus of the protein, interconversion of the 3 different forms of the substrate enzyme without alteration of its enzymatic activity related to the substrate's short half-life in the cell Rattus norvegicus truncated tyrosine aminotransferase + peptide 49 kDa product monomer and 4.5 kDa N-terminal peptide ?
3.4.22.24 tyrosine aminotransferase + H2O 53 kDa substrate monomer, cleavage at the N-terminus of the protein, interconversion of the 3 different forms of the substrate enzyme without alteration of its enzymatic activity Rattus norvegicus truncated tyrosine aminotransferase + peptide 49 kDa product monomer and 4.5 kDa N-terminal peptide ?

Subunits

EC Number Subunits Comment Organism
3.4.22.3 More amino acid sequence sand structure analysis Ficus glabrata
3.4.22.24 monomer 1 x 35000 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.3 6.8
-
-
 Ficus glabrata
3.4.22.24 6.9
-
 at 0°C Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.3 additional information
-
 pH profile Ficus glabrata