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Literature summary extracted from

  • Straeter, N.; Lipscomb, W.N.
    Leucine aminopeptidase (2004), Handbook of Metalloproteins (Messerschmidt, A. ; Huber R. Wieghardt, K. ; Poulos, T. , eds. ) Wiley, 3, 199-207.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 amastatin natural inhibitor Sus scrofa
3.4.11.1 Amino acid hydroxamates
-
 Sus scrofa
3.4.11.1 bestatin natural inhibitor Sus scrofa
3.4.11.1 L-leucylthiol
-
 Sus scrofa
3.4.11.1 leucinal
-
 Sus scrofa
3.4.11.1 leucine phosphonic acid
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 additional information
-
 additional information kinetics Sus scrofa
3.4.11.1 0.06
-
 Leu-Phe Zn2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 1
-
 Gly-Phe Zn2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 2
-
 Leu-4-nitroanilide Mg2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 4.1
-
 Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 8.4
-
 Leu-Gly Mg2+-Zn2+-enzyme form Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ activates Sus scrofa
3.4.11.1 Mn2+ activates Sus scrofa
3.4.11.1 Zn2+ zinc-metallopeptidase, two Zn2+ per protomer Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 native enzyme from kidney Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Gly-Phe + H2O
-
 Sus scrofa Gly + Phe
-
 ?
3.4.11.1 Leu-2-naphthylamide + H2O
-
 Sus scrofa Leu + 2-naphthylamine
-
 ?
3.4.11.1 Leu-4-nitroanilide + H2O
-
 Sus scrofa Leu + 4-nitroaniline
-
 ?
3.4.11.1 Leu-Gly + H2O
-
 Sus scrofa Leu + Gly
-
 ?
3.4.11.1 Leu-Phe + H2O
-
 Sus scrofa Leu + Phe
-
 ?
3.4.11.1 additional information broad substrate specificity Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer homohexamer Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Sus scrofa
3.4.11.1 leucine aminopeptidase
-
 Sus scrofa
3.4.11.1 More the enzyme belongs to the peptidase family M17 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 0.003
-
 Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 0.04
-
 Leu-4-nitroanilide Mg2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 0.5
-
 Gly-Phe Zn2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 270
-
 Leu-Phe Zn2+-Zn2+-enzyme form Sus scrofa
3.4.11.1 1670
-
 Leu-Gly Mg2+-Zn2+-enzyme form Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 9 9.5
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.1 0.0002
-
 amastatin
-
 Sus scrofa