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Literature summary extracted from

  • Taniguchi, A.Y.; Takano, K.
    Purification and properties of b-galactosidase from Tilapia intestine: Digestive enzyme of Tilapia-X (2004), Fish. Sci., 70, 688-694.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cd2+ 1 mM, 39% inhibition Oreochromis niloticus
3.2.1.23 CH2ICOOH 1 mM, 50% inhibition Oreochromis niloticus
3.2.1.23 Cu2+ 1 mM, 16% inhibition Oreochromis niloticus
3.2.1.23 Hg2+ 1 mM, complete inhibition Oreochromis niloticus
3.2.1.23 Mg2+ 1 mM, 31% inhibition Oreochromis niloticus
3.2.1.23 Ni2+ 1 mM, 16% inhibition Oreochromis niloticus
3.2.1.23 p-chloromercuribenzoate 1 mM, complete inhibition Oreochromis niloticus
3.2.1.23 Pb2+ 1 mM, 66% inhibition Oreochromis niloticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 6.25
-
 p-nitrophenyl-beta-D-galactoside
-
 Oreochromis niloticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Al3+ 1 mM, 30% activation Oreochromis niloticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 71000
-
 gel filtration Oreochromis niloticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Oreochromis niloticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Oreochromis niloticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 intestinal juice most of the activity detected in intestinal juice Oreochromis niloticus
-
3.2.1.23 intestine the enzyme is secreted by the upper and middle parts of the intestine Oreochromis niloticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 168.6
-
-
 Oreochromis niloticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 agarose + H2O
-
 Oreochromis niloticus beta-D-galactose + ?
-
 ?
3.2.1.23 galactan + H2O
-
 Oreochromis niloticus beta-D-galactose + ?
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Oreochromis niloticus p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-glucuronide + H2O 60% of the activity with p-nitrophenyl-beta-D-galactoside Oreochromis niloticus p-nitrophenol + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 71000, SDS-PAGE Oreochromis niloticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 40
-
-
 Oreochromis niloticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 30 min, stable below Oreochromis niloticus
3.2.1.23 60
-
 30 min, about 40% loss of activity Oreochromis niloticus
3.2.1.23 70
-
 30 min, about 70% loss of activity Oreochromis niloticus
3.2.1.23 80
-
 30 min, about 90% loss of activity Oreochromis niloticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 5
-
-
 Oreochromis niloticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 3
-
 30 min, 50% loss of activity Oreochromis niloticus
3.2.1.23 4
-
 30 min, 30% loss of activity Oreochromis niloticus
3.2.1.23 5 7 30 min, stable Oreochromis niloticus
3.2.1.23 8
-
 30 min, 30% loss of activity Oreochromis niloticus
3.2.1.23 9
-
 30 min, 50% loss of activity Oreochromis niloticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.23 Oreochromis niloticus electrofocusing, pH-range: 3-10
-
 6.7