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Literature summary extracted from

  • Lamble, H.J.; Milburn, C.C.; Taylor, G.L.; Hough, D.W.; Danson, M.J.
    Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus (2004), FEBS Lett., 576, 133-136.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.140 EGTA enzyme activity is reduced to 24% after dialysis in buffer containing EGTA. Activity is completely recovered in the presence of 10 mM MgCl2 and recovered to 39% and 62% in the presence of MnCl2 and CoCl2, respectively Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.140 0.81
-
 D-galactonate pH 6.0, 70°C Saccharolobus solfataricus
4.2.1.140 1.57
-
 D-gluconate pH 6.0, 70°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.140 Mg2+ Mg2+ has a catalytic role Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.140 44729
-
 1 * 44729, calculated from sequence Saccharolobus solfataricus
4.2.1.140 45000
-
 1 * 45000, SDS-PAGE Saccharolobus solfataricus
4.2.1.140 48000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.140 D-galactonate Saccharolobus solfataricus the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-galactonate Saccharolobus solfataricus P2 the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-gluconate Saccharolobus solfataricus the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.140 D-gluconate Saccharolobus solfataricus P2 the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.140 Saccharolobus solfataricus Q97U27
-
-
4.2.1.140 Saccharolobus solfataricus P2 Q97U27
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.140
-
 Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.140 D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O (2) Saccharolobus solfataricus
a
4.2.1.140 D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O (1) Saccharolobus solfataricus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.140 additional information
-
 13.4 U/mg, pH 6.0, 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.140 D-galactonate the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-galactonate the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-galactonate the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-galactonate the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-gluconate the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.140 D-gluconate the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.140 D-gluconate the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.140 D-gluconate the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.140 monomer 1 * 45000, SDS-PAGE Saccharolobus solfataricus
4.2.1.140 monomer 1 * 44729, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.140 Sso3198
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.140 70
-
 assay at Saccharolobus solfataricus
4.2.1.140 100
-
 above Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.140 95
-
 half-life: 41 min Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.140 1.08
-
 D-galactonate pH 6.0, 70°C Saccharolobus solfataricus
4.2.1.140 10.4
-
 D-gluconate pH 6.0, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.140 6
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
4.2.1.140 physiological function the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.140 1.33
-
 D-galactonate pH 6.0, 70°C Saccharolobus solfataricus
4.2.1.140 6.65
-
 D-gluconate pH 6.0, 70°C Saccharolobus solfataricus