BRENDA - Enzyme Database

Characterization of an oxaloacetate decarboxylase that belongs to the malic enzyme family

Sender, P.D.; Martin, M.G.; Peiru, S.; Magni, C.; FEBS Lett. 570, 217-222 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.1.112
expression in Escherichia coli M15
Lactococcus lactis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.112
2-oxoglutarate
2 mM inhibitor in presence of 10 mM Mn2+, 11% inhibition
Lactococcus lactis
4.1.1.112
Ca2+
2 mM inhibitor in presence of 1 mM Mn2+, 30% inhibition
Lactococcus lactis
4.1.1.112
citrate
0.5 mM inhibitor or 2 mM inhibitor in presence of 10 mM Mn2+, 24% inhibition
Lactococcus lactis
4.1.1.112
Cu2+
0.5 mM inhibitor in presence of 1 mM Mn2+, complete inhibition
Lactococcus lactis
4.1.1.112
EDTA
10 mM, complete inhibition
Lactococcus lactis
4.1.1.112
fumarate
2 mM inhibitor in presence of 10 mM Mn2+, 21% inhibition
Lactococcus lactis
4.1.1.112
malate
2 mM inhibitor in presence of 10 mM Mn2+, 54% inhibition
Lactococcus lactis
4.1.1.112
malonate
0.5 mM inhibitor in presence of 10 mM Mn2+, 84% inhibition
Lactococcus lactis
4.1.1.112
NAD+
2 mM inhibitor in presence of 10 mM Mn2+, complete inhibition
Lactococcus lactis
4.1.1.112
NADH
2 mM inhibitor in presence of 10 mM Mn2+, complete inhibition
Lactococcus lactis
4.1.1.112
Ni2+
2 mM inhibitor in presence of 1 mM Mn2+, 40% inhibition
Lactococcus lactis
4.1.1.112
oxalate
0.5 mM inhibitor in presence of 10 mM Mn2+, 80% inhibition
Lactococcus lactis
4.1.1.112
pyruvate
2 mM inhibitor in presence of 10 mM Mn2+, 8% inhibition
Lactococcus lactis
4.1.1.112
succinate
2 mM inhibitor in presence of 10 mM Mn2+, 23% inhibition
Lactococcus lactis
4.1.1.112
Zn2+
0.5 mM inhibitor in presence of 1 mM Mn2+, complete inhibition
Lactococcus lactis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.112
0.52
-
oxaloacetate
-
Lactococcus lactis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.112
Mg2+
activates
Lactococcus lactis
4.1.1.112
Mn2+
dependent on, maximal activity in presence of 10 mM Mn2+
Lactococcus lactis
4.1.1.112
Ni2+
activates
Lactococcus lactis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.112
Lactococcus lactis
-
CRL264
-
4.1.1.112
Lactococcus lactis CRL264
-
CRL264
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.112
recombinant
Lactococcus lactis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.112
Oxaloacetate
-
664936
Lactococcus lactis
Pyruvate + CO2
-
-
-
ir
4.1.1.112
Oxaloacetate
-
664936
Lactococcus lactis CRL264
Pyruvate + CO2
-
-
-
ir
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.112
50
-
-
Lactococcus lactis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.112
58
-
no activity above
Lactococcus lactis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.112
3.5
-
oxaloacetate
-
Lactococcus lactis
4.1.1.112
20.26
-
oxaloacetate
-
Lactococcus lactis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.112
4.4
-
-
Lactococcus lactis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.112
3.5
5.5
pH 3.5: about 55% of maximal activity, pH 5.5: about 40% of maximal activity
Lactococcus lactis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.112
expression in Escherichia coli M15
Lactococcus lactis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.112
2-oxoglutarate
2 mM inhibitor in presence of 10 mM Mn2+, 11% inhibition
Lactococcus lactis
4.1.1.112
Ca2+
2 mM inhibitor in presence of 1 mM Mn2+, 30% inhibition
Lactococcus lactis
4.1.1.112
citrate
0.5 mM inhibitor or 2 mM inhibitor in presence of 10 mM Mn2+, 24% inhibition
Lactococcus lactis
4.1.1.112
Cu2+
0.5 mM inhibitor in presence of 1 mM Mn2+, complete inhibition
Lactococcus lactis
4.1.1.112
EDTA
10 mM, complete inhibition
Lactococcus lactis
4.1.1.112
fumarate
2 mM inhibitor in presence of 10 mM Mn2+, 21% inhibition
Lactococcus lactis
4.1.1.112
malate
2 mM inhibitor in presence of 10 mM Mn2+, 54% inhibition
Lactococcus lactis
4.1.1.112
malonate
0.5 mM inhibitor in presence of 10 mM Mn2+, 84% inhibition
Lactococcus lactis
4.1.1.112
NAD+
2 mM inhibitor in presence of 10 mM Mn2+, complete inhibition
Lactococcus lactis
4.1.1.112
NADH
2 mM inhibitor in presence of 10 mM Mn2+, complete inhibition
Lactococcus lactis
4.1.1.112
Ni2+
2 mM inhibitor in presence of 1 mM Mn2+, 40% inhibition
Lactococcus lactis
4.1.1.112
oxalate
0.5 mM inhibitor in presence of 10 mM Mn2+, 80% inhibition
Lactococcus lactis
4.1.1.112
pyruvate
2 mM inhibitor in presence of 10 mM Mn2+, 8% inhibition
Lactococcus lactis
4.1.1.112
succinate
2 mM inhibitor in presence of 10 mM Mn2+, 23% inhibition
Lactococcus lactis
4.1.1.112
Zn2+
0.5 mM inhibitor in presence of 1 mM Mn2+, complete inhibition
Lactococcus lactis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.112
0.52
-
oxaloacetate
-
Lactococcus lactis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.112
Mg2+
activates
Lactococcus lactis
4.1.1.112
Mn2+
dependent on, maximal activity in presence of 10 mM Mn2+
Lactococcus lactis
4.1.1.112
Ni2+
activates
Lactococcus lactis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.112
recombinant
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.112
Oxaloacetate
-
664936
Lactococcus lactis
Pyruvate + CO2
-
-
-
ir
4.1.1.112
Oxaloacetate
-
664936
Lactococcus lactis CRL264
Pyruvate + CO2
-
-
-
ir
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.112
50
-
-
Lactococcus lactis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.1.1.112
58
-
no activity above
Lactococcus lactis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.112
3.5
-
oxaloacetate
-
Lactococcus lactis
4.1.1.112
20.26
-
oxaloacetate
-
Lactococcus lactis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.112
4.4
-
-
Lactococcus lactis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.112
3.5
5.5
pH 3.5: about 55% of maximal activity, pH 5.5: about 40% of maximal activity
Lactococcus lactis