Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chon, H.; Tadokoro, T.; Ohtani, N.; Koga, Y.; Takano, K.; Kanaya, S.
    Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H (2006), FEBS J., 273, 2264-2275.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.4 gene rnhB, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain MIC2067(DE3), a rnhA/rnhB double mutant strain, as 60% soluble and 40% insoluble protein Shewanella sp.

General Stability

EC Number General Stability Organism
3.1.26.4 denaturation kinetics, midpoint at about 1.6 M urea and 5°C Shewanella sp.
3.1.26.4 denaturation kinetics, midpoint at about 1.8 M urea and 5°C Escherichia coli
3.1.26.4 metal ion and substrate stabilize the enzyme Escherichia coli
3.1.26.4 metal ion and substrate stabilize the enzyme Shewanella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.4 0.071
-
 M13 DNA-RNA hybrid pH 8.5, 15°C, recombinant enzyme Shewanella sp.
3.1.26.4 0.075
-
 M13 DNA-RNA hybrid pH 8.5, 30°C, recombinant enzyme Shewanella sp.
3.1.26.4 0.26
-
 M13 DNA-RNA hybrid pH 8.5, 15°C or 30°C, recombinant enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Co2+ activates Escherichia coli
3.1.26.4 Co2+ activates, best at 0.5 mM Shewanella sp.
3.1.26.4 KCl activates best at 110 mM, preferred to NaCl Shewanella sp.
3.1.26.4 KCl equally activating as NaCl Escherichia coli
3.1.26.4 Mg2+ activates Escherichia coli
3.1.26.4 Mg2+ activates, best at 5 mM Shewanella sp.
3.1.26.4 Mn2+ activates Escherichia coli
3.1.26.4 Mn2+ activates, best at 1 mM, strongly preferred divalent cation Shewanella sp.
3.1.26.4 additional information no activation by Ca2+, Zn2+, Ba2+, Ni2+, Cu2+, Fe2+, and Sr2+ Escherichia coli
3.1.26.4 additional information no activation by Ca2+, Zn2+, Ba2+, Ni2+, Cu2+, Fe2+, and Sr2+ Shewanella sp.
3.1.26.4 NaCl activates best at 60 mM Shewanella sp.
3.1.26.4 NaCl equally activating as KCl Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 25000
-
 recombinant enzyme, gel filtration Shewanella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Escherichia coli P10442 gene rnhB
-
3.1.26.4 Shewanella sp. Q25C12 psychrotrophic bacterium, gene rnhB
-
3.1.26.4 Shewanella sp. SIB1 Q25C12 psychrotrophic bacterium, gene rnhB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 recombinant enzyme from Escherichia coli strain MIC2067(DE3) to homogeneity by heparin affinity chromatography and gel filtration Shewanella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 0.31
-
 15°C, pH 8.5, purified recombinant enzyme Escherichia coli
3.1.26.4 1.1
-
 30°C, pH 8.5, purified recombinant enzyme Escherichia coli
3.1.26.4 7.6
-
 15°C, pH 8.5, purified recombinant enzyme Shewanella sp.
3.1.26.4 22
-
 30°C, pH 8.5, purified recombinant enzyme Shewanella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 DNA-RNA hybrid + H2O
-
 Escherichia coli ?
-
 ?
3.1.26.4 DNA-RNA hybrid + H2O
-
 Shewanella sp. ?
-
 ?
3.1.26.4 DNA-RNA hybrid + H2O
-
 Shewanella sp. SIB1 ?
-
 ?
3.1.26.4 M13 DNA-RNA hybrid + H2O
-
 Escherichia coli ?
-
 ?
3.1.26.4 M13 DNA-RNA hybrid + H2O
-
 Shewanella sp. ?
-
 ?
3.1.26.4 M13 DNA-RNA hybrid + H2O
-
 Shewanella sp. SIB1 ?
-
 ?
3.1.26.4 additional information substrate cleavage mode of the enzyme, cleavage site specificity Escherichia coli ?
-
 ?
3.1.26.4 additional information substrate cleavage mode of the enzyme, cleavage site specificity Shewanella sp. ?
-
 ?
3.1.26.4 additional information substrate cleavage mode of the enzyme, cleavage site specificity Shewanella sp. SIB1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 monomer 1 * 25000, recombinant enzyme, SDS-PAGE Shewanella sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 RNase HII
-
 Escherichia coli
3.1.26.4 RNase HII
-
 Shewanella sp.
3.1.26.4 RnhB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.4 40
-
-
 Shewanella sp.
3.1.26.4 45
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.26.4 15 60
-
 Escherichia coli
3.1.26.4 15 60
-
 Shewanella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.26.4 30
-
 half-life about 30 min Shewanella sp.
3.1.26.4 30
-
 half-life about 45 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 10
-
-
 Escherichia coli
3.1.26.4 10
-
-
 Shewanella sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.26.4 7.1 12
-
 Escherichia coli
3.1.26.4 7.1 12
-
 Shewanella sp.